Identification of muscle necrosis in the mdx mouse model of Duchenne muscular dystrophy using three-dimensional optical coherence tomography

Blake R. Klyen, Thea Shavlakadze, Hannah G. Radley-Crabb, Miranda D. Grounds, David D. Sampson

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Three-dimensional optical coherence tomography (3D-OCT) was used to image the structure and pathology of skeletal muscle tissue from the treadmill-exercised mdx mouse model of human Duchenne muscular dystrophy. Optical coherence tomography (OCT) images of excised muscle samples were compared with coregistered hematoxylin and eosin-stained and Evans blue dye fluorescence histology. We show, for the first time, structural 3D-OCT images of skeletal muscle dystropathology well correlated with co-located histology. OCT could identify morphological features of interest and necrotic lesions within the muscle tissue samples based on intrinsic optical contrast. These findings demonstrate the utility of 3D-OCT for the evaluation of small-animal skeletal muscle morphology and pathology, particularly for studies of mouse models of muscular dystrophy. 

Original languageEnglish
Article number076013
Number of pages9
JournalJournal of Biomedical Optics
Issue number7
Publication statusPublished - Jul 2011
Externally publishedYes


  • Duchenne muscular dystrophy
  • Evans blue dye
  • Mdx mouse
  • Necrotic lesion
  • Optical coherence tomography
  • Skeletal muscle
  • Small-animal imaging
  • Three-dimensional imaging

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