Identification of muscle necrosis in the mdx mouse model of Duchenne muscular dystrophy using three-dimensional optical coherence tomography

Blake R. Klyen, Thea Shavlakadze, Hannah G. Radley-Crabb, Miranda D. Grounds, David D. Sampson

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Abstract

Three-dimensional optical coherence tomography (3D-OCT) was used to image the structure and pathology of skeletal muscle tissue from the treadmill-exercised mdx mouse model of human Duchenne muscular dystrophy. Optical coherence tomography (OCT) images of excised muscle samples were compared with coregistered hematoxylin and eosin-stained and Evans blue dye fluorescence histology. We show, for the first time, structural 3D-OCT images of skeletal muscle dystropathology well correlated with co-located histology. OCT could identify morphological features of interest and necrotic lesions within the muscle tissue samples based on intrinsic optical contrast. These findings demonstrate the utility of 3D-OCT for the evaluation of small-animal skeletal muscle morphology and pathology, particularly for studies of mouse models of muscular dystrophy. 

Original languageEnglish
Article number076013
Number of pages9
JournalJournal of Biomedical Optics
Volume16
Issue number7
DOIs
Publication statusPublished - Jul 2011
Externally publishedYes

Keywords

  • Duchenne muscular dystrophy
  • Evans blue dye
  • Mdx mouse
  • Necrotic lesion
  • Optical coherence tomography
  • Skeletal muscle
  • Small-animal imaging
  • Three-dimensional imaging

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