Identification of Metabolically Distinct Adipocyte Progenitor Cells in Human Adipose Tissues

Arthe Raajendiran; Geraldine Ooi; Jackie Bayliss; Paul E. O'Brien; Ralf B. Schittenhelm; Ashlee K. Clark; Renea A. Taylor; Matthew S. Rodeheffer; Paul R. Burton; Matthew J. Watt

doi:10.1016/j.celrep.2019.04.010

Identification of Metabolically Distinct Adipocyte Progenitor Cells in Human Adipose Tissues

Arthe Raajendiran, Geraldine Ooi, Jackie Bayliss, Paul E. O'Brien, Ralf B. Schittenhelm, Ashlee K. Clark, Renea A. Taylor, Matthew S. Rodeheffer, Paul R. Burton, Matthew J. Watt

Research output: Contribution to journal › Article › Research › peer-review

117 Citations (Scopus)

Abstract

Adipocyte progenitor cells (APCs) provide the reservoir of regenerative cells to produce new adipocytes, although their identity in humans remains elusive. Using FACS analysis, gene expression profiling, and metabolic and proteomic analyses, we identified three APC subtypes in human white adipose tissues. The APC subtypes are molecularly distinct but possess similar proliferative and adipogenic capacities. Adipocytes derived from APCs with high CD34 expression exhibit exceedingly high rates of lipid flux compared with APCs with low or no CD34 expression, while adipocytes produced from CD34 ⁻ APCs display beige-like adipocyte properties and a unique endocrine profile. APCs were more abundant in gluteofemoral compared with abdominal subcutaneous and omental adipose tissues, and the distribution of APC subtypes varies between depots and in patients with type 2 diabetes. These findings provide a mechanistic explanation for the heterogeneity of human white adipose tissue and a potential basis for dysregulated adipocyte function in type 2 diabetes.

Original language	English
Pages (from-to)	1528-1540
Number of pages	13
Journal	Cell Reports
Volume	27
Issue number	5
DOIs	https://doi.org/10.1016/j.celrep.2019.04.010
Publication status	Published - 30 Apr 2019

Keywords

adipocyte progenitor cell
adipogenesis
adipokine
adipose tissue
beige adipocyte
lipid metabolism
lipolysis
obesity
type 2 diabetes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.celrep.2019.04.010

271884261-oaFinal published version, 3.41 MB

Cite this

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title = "Identification of Metabolically Distinct Adipocyte Progenitor Cells in Human Adipose Tissues",

abstract = " Adipocyte progenitor cells (APCs) provide the reservoir of regenerative cells to produce new adipocytes, although their identity in humans remains elusive. Using FACS analysis, gene expression profiling, and metabolic and proteomic analyses, we identified three APC subtypes in human white adipose tissues. The APC subtypes are molecularly distinct but possess similar proliferative and adipogenic capacities. Adipocytes derived from APCs with high CD34 expression exhibit exceedingly high rates of lipid flux compared with APCs with low or no CD34 expression, while adipocytes produced from CD34 − APCs display beige-like adipocyte properties and a unique endocrine profile. APCs were more abundant in gluteofemoral compared with abdominal subcutaneous and omental adipose tissues, and the distribution of APC subtypes varies between depots and in patients with type 2 diabetes. These findings provide a mechanistic explanation for the heterogeneity of human white adipose tissue and a potential basis for dysregulated adipocyte function in type 2 diabetes. ",

keywords = "adipocyte progenitor cell, adipogenesis, adipokine, adipose tissue, beige adipocyte, lipid metabolism, lipolysis, obesity, type 2 diabetes",

author = "Arthe Raajendiran and Geraldine Ooi and Jackie Bayliss and O'Brien, {Paul E.} and Schittenhelm, {Ralf B.} and Clark, {Ashlee K.} and Taylor, {Renea A.} and Rodeheffer, {Matthew S.} and Burton, {Paul R.} and Watt, {Matthew J.}",

year = "2019",

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doi = "10.1016/j.celrep.2019.04.010",

language = "English",

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AU - Raajendiran, Arthe

AU - Ooi, Geraldine

AU - Bayliss, Jackie

AU - O'Brien, Paul E.

AU - Schittenhelm, Ralf B.

AU - Clark, Ashlee K.

AU - Taylor, Renea A.

AU - Rodeheffer, Matthew S.

AU - Burton, Paul R.

AU - Watt, Matthew J.

PY - 2019/4/30

Y1 - 2019/4/30

N2 - Adipocyte progenitor cells (APCs) provide the reservoir of regenerative cells to produce new adipocytes, although their identity in humans remains elusive. Using FACS analysis, gene expression profiling, and metabolic and proteomic analyses, we identified three APC subtypes in human white adipose tissues. The APC subtypes are molecularly distinct but possess similar proliferative and adipogenic capacities. Adipocytes derived from APCs with high CD34 expression exhibit exceedingly high rates of lipid flux compared with APCs with low or no CD34 expression, while adipocytes produced from CD34 − APCs display beige-like adipocyte properties and a unique endocrine profile. APCs were more abundant in gluteofemoral compared with abdominal subcutaneous and omental adipose tissues, and the distribution of APC subtypes varies between depots and in patients with type 2 diabetes. These findings provide a mechanistic explanation for the heterogeneity of human white adipose tissue and a potential basis for dysregulated adipocyte function in type 2 diabetes.

AB - Adipocyte progenitor cells (APCs) provide the reservoir of regenerative cells to produce new adipocytes, although their identity in humans remains elusive. Using FACS analysis, gene expression profiling, and metabolic and proteomic analyses, we identified three APC subtypes in human white adipose tissues. The APC subtypes are molecularly distinct but possess similar proliferative and adipogenic capacities. Adipocytes derived from APCs with high CD34 expression exhibit exceedingly high rates of lipid flux compared with APCs with low or no CD34 expression, while adipocytes produced from CD34 − APCs display beige-like adipocyte properties and a unique endocrine profile. APCs were more abundant in gluteofemoral compared with abdominal subcutaneous and omental adipose tissues, and the distribution of APC subtypes varies between depots and in patients with type 2 diabetes. These findings provide a mechanistic explanation for the heterogeneity of human white adipose tissue and a potential basis for dysregulated adipocyte function in type 2 diabetes.

KW - adipocyte progenitor cell

KW - adipogenesis

KW - adipokine

KW - adipose tissue

KW - beige adipocyte

KW - lipid metabolism

KW - lipolysis

KW - obesity

KW - type 2 diabetes

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DO - 10.1016/j.celrep.2019.04.010

M3 - Article

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SN - 2211-1247

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JO - Cell Reports

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Identification of Metabolically Distinct Adipocyte Progenitor Cells in Human Adipose Tissues

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Deciphering the metabolic and endocrine profile of healthy adipocytes

NHMRC Research Fellowship

FlowCore

Monash Micro Imaging

Monash Proteomics & Metabolomics Facility

Cite this

Identification of Metabolically Distinct Adipocyte Progenitor Cells in Human Adipose Tissues

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Projects

Deciphering the metabolic and endocrine profile of healthy adipocytes

NHMRC Research Fellowship

Equipment

FlowCore

Monash Micro Imaging

Monash Proteomics & Metabolomics Facility

Cite this