Identification of genes essential for leptospirosis

Thanatchaporn Bartpho, Gerald L Murray

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

2 Citations (Scopus)


The development of methods for the construction of defined mutants of pathogenic Leptospira has been a breakthrough in the study of leptospiral virulence. These methods have allowed the identification of genes essential for infection in animal models. This chapter describes methods for random transposon mutagenesis of pathogenic leptospires, identification of transposon insertion sites using direct sequencing from genomic DNA and a nested PCR utilizing degenerate oligonucleotides, and methods for testing mutant attenuation in the hamster model of infection.
Original languageEnglish
Title of host publicationGene Essentiality
Subtitle of host publicationMethods and Protocols
EditorsLong Jason Lu
Place of PublicationNew York NY USA
PublisherHumana Press
Number of pages12
ISBN (Electronic)978-1-4939-2398-4
ISBN (Print)978-1-4939-2397-7
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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