Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase

Gln Sheng Feng, Karen Chong, Aseem Kumar, Bryan R.G. Williams

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144 Citations (Scopus)

Abstract

The double-stranded RNA (dsRNA)-binding domain of the human p68 kinase has been localized to the N-terminal half of the enzyme by using progressive deletion analysis and in vitro binding assays. To further define the domains responsible for binding to dsRNA, we cloned the mouse dsRNA-activated p65 kinase and used sequence alignment to identify conserved domains in the N-terminal region. Deletions in either of two 12-amino-acid-long and arginine- or lysine-rich regions abrogated binding to dsRNA. Moreover, in an in vivo growth inhibition assay in the yeast Saccharomyces cerevisiae, these mutants failed to exhibit a slow-growth phenotype.

Original languageEnglish
Pages (from-to)5447-5451
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number12
DOIs
Publication statusPublished - 1 Jan 1992
Externally publishedYes

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