TY - JOUR
T1 - Identification of Bacillus spores using clustering of principal components of fluorescence data
AU - Kunnil, Joseph
AU - Sarasanandarajah, Sivananthan
AU - Chacko, Easaw
AU - Swartz, Barry
AU - Reinisch, Lou
PY - 2005/9
Y1 - 2005/9
N2 - We have measured the fluorescence spectra of three different bacterial spores (B. globigii, B. cereus, and B. papillae) mixed with one of three different samples of domestic dust and using five excitation wavelengths. The data are analyzed with principal component analysis and clustered with partition around medoids method. We are able to correctly identify each of the samples, even with the dust involved. We also measured a separate preparation of B. globigii, ovalbumin, and corn smut fungal spores. All the B. globigii samples showed excellent clustering. The ovalbumin and corn smut spores showed weak or ambiguous clustering with B. cereus. While fluorescence combined with principal component analysis and clustering techniques shows strong promise to correctly identify bacterial spores, additional work is necessary to discriminate the fluorescence signals from other biological samples.
AB - We have measured the fluorescence spectra of three different bacterial spores (B. globigii, B. cereus, and B. papillae) mixed with one of three different samples of domestic dust and using five excitation wavelengths. The data are analyzed with principal component analysis and clustered with partition around medoids method. We are able to correctly identify each of the samples, even with the dust involved. We also measured a separate preparation of B. globigii, ovalbumin, and corn smut fungal spores. All the B. globigii samples showed excellent clustering. The ovalbumin and corn smut spores showed weak or ambiguous clustering with B. cereus. While fluorescence combined with principal component analysis and clustering techniques shows strong promise to correctly identify bacterial spores, additional work is necessary to discriminate the fluorescence signals from other biological samples.
UR - http://www.scopus.com/inward/record.url?scp=27744499782&partnerID=8YFLogxK
U2 - 10.1080/02786820500295255
DO - 10.1080/02786820500295255
M3 - Article
AN - SCOPUS:27744499782
SN - 0278-6826
VL - 39
SP - 842
EP - 848
JO - Aerosol Science and Technology
JF - Aerosol Science and Technology
IS - 9
ER -