Identification of an interaction between SOX9 and HSP70

Owen J. Marshall, Vincent R. Harley

Research output: Contribution to journalArticleResearchpeer-review

27 Citations (Scopus)

Abstract

The campomelic dysplasia/autosomal sex reversal protein SOX9 is an important developmental transcription factor, required for correct bone and testis formation. Through in vitro and in vivo studies we have identified the heat shock protein HSP70 as an interacting partner for SOX9 in chondrocyte and testicular cell lines. HSP70 forms a ternary complex with DNA-bound SOX9. The interaction between HSP70 and SOX9 is ATP-independent and involves a highly conserved region of SOX9 hitherto of unknown function and the C-terminal region of HSP70. Our results implicate HSP70-SOX9 interactions in the assembly of multi-protein complexes during SOX9-mediated transcription.

Original languageEnglish
Pages (from-to)75-80
Number of pages6
JournalFEBS Letters
Volume496
Issue number2-3
DOIs
Publication statusPublished - 11 May 2001
Externally publishedYes

Keywords

  • Campomelic dysplasia
  • HSP70
  • Protein-protein interaction
  • SOX9

Cite this