Identification of a role for nucleolin in rabies virus infection

Sibil Oksayan, Jovan Nikolic, C. T. David, Danielle Blondel, David A Jans, Gregory W Moseley

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

Rabies virus replicates in the cytoplasm of host cells, but rabies virus phosphoprotein (P-protein) undergoes active nucleocytoplasmic trafficking. Here we show that the largely nuclear P-protein isoform P3 can localise to nucleoli, and forms specific interactions with nucleolin. Importantly, depletion of nucleolin expression inhibits viral protein expression and infectious virus production by infected cells. This provides the first evidence that lyssaviruses interact with nucleolin, and that nucleolin is important to lyssavirus infection.
Original languageEnglish
Pages (from-to)1939 - 1943
Number of pages5
JournalJournal of Virology
Volume89
Issue number3
DOIs
Publication statusPublished - 2015

Cite this

Oksayan, Sibil ; Nikolic, Jovan ; David, C. T. ; Blondel, Danielle ; Jans, David A ; Moseley, Gregory W. / Identification of a role for nucleolin in rabies virus infection. In: Journal of Virology. 2015 ; Vol. 89, No. 3. pp. 1939 - 1943.
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Identification of a role for nucleolin in rabies virus infection. / Oksayan, Sibil; Nikolic, Jovan; David, C. T.; Blondel, Danielle; Jans, David A; Moseley, Gregory W.

In: Journal of Virology, Vol. 89, No. 3, 2015, p. 1939 - 1943.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Identification of a role for nucleolin in rabies virus infection

AU - Oksayan, Sibil

AU - Nikolic, Jovan

AU - David, C. T.

AU - Blondel, Danielle

AU - Jans, David A

AU - Moseley, Gregory W

PY - 2015

Y1 - 2015

N2 - Rabies virus replicates in the cytoplasm of host cells, but rabies virus phosphoprotein (P-protein) undergoes active nucleocytoplasmic trafficking. Here we show that the largely nuclear P-protein isoform P3 can localise to nucleoli, and forms specific interactions with nucleolin. Importantly, depletion of nucleolin expression inhibits viral protein expression and infectious virus production by infected cells. This provides the first evidence that lyssaviruses interact with nucleolin, and that nucleolin is important to lyssavirus infection.

AB - Rabies virus replicates in the cytoplasm of host cells, but rabies virus phosphoprotein (P-protein) undergoes active nucleocytoplasmic trafficking. Here we show that the largely nuclear P-protein isoform P3 can localise to nucleoli, and forms specific interactions with nucleolin. Importantly, depletion of nucleolin expression inhibits viral protein expression and infectious virus production by infected cells. This provides the first evidence that lyssaviruses interact with nucleolin, and that nucleolin is important to lyssavirus infection.

UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300734/pdf/zjv1939.pdf

U2 - 10.1128/JVI.03320-14

DO - 10.1128/JVI.03320-14

M3 - Article

VL - 89

SP - 1939

EP - 1943

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

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