Identification of a novel autoantibody against self-vimentin specific in secondary Sjögren's syndrome

Yu Hui Li, Ya Ping Gao, Jie Dong, Lian Jie Shi, Xiao Lin Sun, Ru Li, Xue Wu Zhang, Yu Liu, Li Long, Jing He, Qun Jie Zhong, Eric Morand, Guang Yang, Zhan Guo Li

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Background: Sjögren's syndrome (SS) is a primary autoimmune disease (pSS) or secondarily associated with other autoimmune diseases (sSS). The mechanisms underlying immune dysregulation in this syndrome remain unknown, and clinically it is difficult to diagnose owing to a lack of specific biomarkers. Methods: We extracted immunoglobulins (Igs) from the sera of patients with sSS associated with rheumatoid arthritis (RA) and used them to screen a phage display library of peptides with random sequences. Results: Our results show that an sSS-specific peptide, designated 3S-P, was recognized by sera of 68.2% (60 of 88) patients with sSS, 66.2% of patients with RA-sSS, and 76.5% of patients with systemic lupus erythematosus (SLE)-sSS. The anti-3S-P antibody was scarcely found in patients with pSS (1.8%), RA (1.3%), SLE (4.2%), ankylosing spondylitis (0%), and gout (3.3%), as well as in healthy donors (2%). The 3S-P-binding Igs (antibodies) were used to identify antigens from salivary glands and synovial tissues from patients with sSS. A putative target autoantigen expressed in the synovium and salivary gland recognized by anti-3S-P antibody was identified as self-vimentin. Conclusions: This novel autoantibody is highly specific in the diagnosis of sSS, and the underlying molecular mechanism of the disease might be epitope spreading involved with vimentin.

Original languageEnglish
Article number30
Number of pages8
JournalArthritis Research and Therapy
Volume20
Issue number1
DOIs
Publication statusPublished - 12 Feb 2018

Keywords

  • Autoantigen
  • Biomarker
  • Sjögren's syndrome
  • Vimentin

Cite this

Li, Yu Hui ; Gao, Ya Ping ; Dong, Jie ; Shi, Lian Jie ; Sun, Xiao Lin ; Li, Ru ; Zhang, Xue Wu ; Liu, Yu ; Long, Li ; He, Jing ; Zhong, Qun Jie ; Morand, Eric ; Yang, Guang ; Li, Zhan Guo. / Identification of a novel autoantibody against self-vimentin specific in secondary Sjögren's syndrome. In: Arthritis Research and Therapy. 2018 ; Vol. 20, No. 1.
@article{b42fd741b481442e88232c4c59cdd2b3,
title = "Identification of a novel autoantibody against self-vimentin specific in secondary Sj{\"o}gren's syndrome",
abstract = "Background: Sj{\"o}gren's syndrome (SS) is a primary autoimmune disease (pSS) or secondarily associated with other autoimmune diseases (sSS). The mechanisms underlying immune dysregulation in this syndrome remain unknown, and clinically it is difficult to diagnose owing to a lack of specific biomarkers. Methods: We extracted immunoglobulins (Igs) from the sera of patients with sSS associated with rheumatoid arthritis (RA) and used them to screen a phage display library of peptides with random sequences. Results: Our results show that an sSS-specific peptide, designated 3S-P, was recognized by sera of 68.2{\%} (60 of 88) patients with sSS, 66.2{\%} of patients with RA-sSS, and 76.5{\%} of patients with systemic lupus erythematosus (SLE)-sSS. The anti-3S-P antibody was scarcely found in patients with pSS (1.8{\%}), RA (1.3{\%}), SLE (4.2{\%}), ankylosing spondylitis (0{\%}), and gout (3.3{\%}), as well as in healthy donors (2{\%}). The 3S-P-binding Igs (antibodies) were used to identify antigens from salivary glands and synovial tissues from patients with sSS. A putative target autoantigen expressed in the synovium and salivary gland recognized by anti-3S-P antibody was identified as self-vimentin. Conclusions: This novel autoantibody is highly specific in the diagnosis of sSS, and the underlying molecular mechanism of the disease might be epitope spreading involved with vimentin.",
keywords = "Autoantigen, Biomarker, Sj{\"o}gren's syndrome, Vimentin",
author = "Li, {Yu Hui} and Gao, {Ya Ping} and Jie Dong and Shi, {Lian Jie} and Sun, {Xiao Lin} and Ru Li and Zhang, {Xue Wu} and Yu Liu and Li Long and Jing He and Zhong, {Qun Jie} and Eric Morand and Guang Yang and Li, {Zhan Guo}",
year = "2018",
month = "2",
day = "12",
doi = "10.1186/s13075-017-1508-5",
language = "English",
volume = "20",
journal = "Arthritis Research and Therapy",
issn = "1478-6354",
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Li, YH, Gao, YP, Dong, J, Shi, LJ, Sun, XL, Li, R, Zhang, XW, Liu, Y, Long, L, He, J, Zhong, QJ, Morand, E, Yang, G & Li, ZG 2018, 'Identification of a novel autoantibody against self-vimentin specific in secondary Sjögren's syndrome', Arthritis Research and Therapy, vol. 20, no. 1, 30. https://doi.org/10.1186/s13075-017-1508-5

Identification of a novel autoantibody against self-vimentin specific in secondary Sjögren's syndrome. / Li, Yu Hui; Gao, Ya Ping; Dong, Jie; Shi, Lian Jie; Sun, Xiao Lin; Li, Ru; Zhang, Xue Wu; Liu, Yu; Long, Li; He, Jing; Zhong, Qun Jie; Morand, Eric; Yang, Guang; Li, Zhan Guo.

In: Arthritis Research and Therapy, Vol. 20, No. 1, 30, 12.02.2018.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Identification of a novel autoantibody against self-vimentin specific in secondary Sjögren's syndrome

AU - Li, Yu Hui

AU - Gao, Ya Ping

AU - Dong, Jie

AU - Shi, Lian Jie

AU - Sun, Xiao Lin

AU - Li, Ru

AU - Zhang, Xue Wu

AU - Liu, Yu

AU - Long, Li

AU - He, Jing

AU - Zhong, Qun Jie

AU - Morand, Eric

AU - Yang, Guang

AU - Li, Zhan Guo

PY - 2018/2/12

Y1 - 2018/2/12

N2 - Background: Sjögren's syndrome (SS) is a primary autoimmune disease (pSS) or secondarily associated with other autoimmune diseases (sSS). The mechanisms underlying immune dysregulation in this syndrome remain unknown, and clinically it is difficult to diagnose owing to a lack of specific biomarkers. Methods: We extracted immunoglobulins (Igs) from the sera of patients with sSS associated with rheumatoid arthritis (RA) and used them to screen a phage display library of peptides with random sequences. Results: Our results show that an sSS-specific peptide, designated 3S-P, was recognized by sera of 68.2% (60 of 88) patients with sSS, 66.2% of patients with RA-sSS, and 76.5% of patients with systemic lupus erythematosus (SLE)-sSS. The anti-3S-P antibody was scarcely found in patients with pSS (1.8%), RA (1.3%), SLE (4.2%), ankylosing spondylitis (0%), and gout (3.3%), as well as in healthy donors (2%). The 3S-P-binding Igs (antibodies) were used to identify antigens from salivary glands and synovial tissues from patients with sSS. A putative target autoantigen expressed in the synovium and salivary gland recognized by anti-3S-P antibody was identified as self-vimentin. Conclusions: This novel autoantibody is highly specific in the diagnosis of sSS, and the underlying molecular mechanism of the disease might be epitope spreading involved with vimentin.

AB - Background: Sjögren's syndrome (SS) is a primary autoimmune disease (pSS) or secondarily associated with other autoimmune diseases (sSS). The mechanisms underlying immune dysregulation in this syndrome remain unknown, and clinically it is difficult to diagnose owing to a lack of specific biomarkers. Methods: We extracted immunoglobulins (Igs) from the sera of patients with sSS associated with rheumatoid arthritis (RA) and used them to screen a phage display library of peptides with random sequences. Results: Our results show that an sSS-specific peptide, designated 3S-P, was recognized by sera of 68.2% (60 of 88) patients with sSS, 66.2% of patients with RA-sSS, and 76.5% of patients with systemic lupus erythematosus (SLE)-sSS. The anti-3S-P antibody was scarcely found in patients with pSS (1.8%), RA (1.3%), SLE (4.2%), ankylosing spondylitis (0%), and gout (3.3%), as well as in healthy donors (2%). The 3S-P-binding Igs (antibodies) were used to identify antigens from salivary glands and synovial tissues from patients with sSS. A putative target autoantigen expressed in the synovium and salivary gland recognized by anti-3S-P antibody was identified as self-vimentin. Conclusions: This novel autoantibody is highly specific in the diagnosis of sSS, and the underlying molecular mechanism of the disease might be epitope spreading involved with vimentin.

KW - Autoantigen

KW - Biomarker

KW - Sjögren's syndrome

KW - Vimentin

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U2 - 10.1186/s13075-017-1508-5

DO - 10.1186/s13075-017-1508-5

M3 - Article

VL - 20

JO - Arthritis Research and Therapy

JF - Arthritis Research and Therapy

SN - 1478-6354

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