Identification of a membrane protein required for lipomannan maturation and lipoarabinomannan synthesis in corynebacterineae

Tamaryn J. Cashmore, Stephan Klatt, Yoshiki Yamaryo-Botte, Rajini Brammananth, Arek K. Rainczuk, Malcolm J McConville, Paul K. Crellin, Ross L. Coppel

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Mycobacterium tuberculosis and related Corynebacterineae synthesize a family of lipomannans (LM) and lipoarabinomannans (LAM) that are abundant components of the multilaminate cell wall and essential virulence factors in pathogenic species. Here we describe a new membrane protein, highly conserved in all Corynebacterineae, that is required for synthesis of fulllength LM and LAM. Deletion of the Corynebacterium glutamicum NCgl2760 gene resulted in a complete loss of mature LM/LAM and the appearance of a truncated LM (t-LM). Complementation of the mutant with the NCgl2760 gene fully restored LM/LAM synthesis. Structural studies, including monosaccharide analysis, methylation linkage analysis, and mass spectrometry of native LM species, indicated that the ΔNCgl2760 t-LM comprised a series of short LM species (8-27 residues long) containing an α1-6-linked mannose backbone with greatly reduced α1-2-mannose side chains and no arabinose caps. The structure of the ΔNCgl2760 t-LM was similar to that of the t-LM produced by a C. glutamicum mutant lacking the mptA gene, encoding a membrane α1-6-mannosyltransferase involved in extending the α1-6-mannan backbone of LM intermediates. Interestingly, NCgl2760 lacks any motifs or homology to other proteins of known function. Attempts to delete the NCgl2760 orthologue in Mycobacterium smegmatis were unsuccessful, consistent with previous studies indicating that the M. tuberculosis orthologue, Rv0227c, is an essential gene. Together, these data suggest that NCgl2760/ Rv0227c plays a critical role in the elongation of the mannan backbone of mycobacterial and corynebacterial LM, further highlighting the complexity of lipoglycan pathways of Corynebacterineae.

Original languageEnglish
Pages (from-to)4976-4986
Number of pages11
JournalJournal of Biological Chemistry
Volume292
Issue number12
DOIs
Publication statusPublished - 24 Mar 2017

Keywords

  • cell wall
  • glycolipid
  • lipid synthesis
  • membrane protein
  • Mycobacterium tuerculosis
  • Coryneacterium glutamicum
  • lipoarabinomannan
  • lipomannan

Cite this

Cashmore, Tamaryn J. ; Klatt, Stephan ; Yamaryo-Botte, Yoshiki ; Brammananth, Rajini ; Rainczuk, Arek K. ; McConville, Malcolm J ; Crellin, Paul K. ; Coppel, Ross L. / Identification of a membrane protein required for lipomannan maturation and lipoarabinomannan synthesis in corynebacterineae. In: Journal of Biological Chemistry. 2017 ; Vol. 292, No. 12. pp. 4976-4986.
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abstract = "Mycobacterium tuberculosis and related Corynebacterineae synthesize a family of lipomannans (LM) and lipoarabinomannans (LAM) that are abundant components of the multilaminate cell wall and essential virulence factors in pathogenic species. Here we describe a new membrane protein, highly conserved in all Corynebacterineae, that is required for synthesis of fulllength LM and LAM. Deletion of the Corynebacterium glutamicum NCgl2760 gene resulted in a complete loss of mature LM/LAM and the appearance of a truncated LM (t-LM). Complementation of the mutant with the NCgl2760 gene fully restored LM/LAM synthesis. Structural studies, including monosaccharide analysis, methylation linkage analysis, and mass spectrometry of native LM species, indicated that the ΔNCgl2760 t-LM comprised a series of short LM species (8-27 residues long) containing an α1-6-linked mannose backbone with greatly reduced α1-2-mannose side chains and no arabinose caps. The structure of the ΔNCgl2760 t-LM was similar to that of the t-LM produced by a C. glutamicum mutant lacking the mptA gene, encoding a membrane α1-6-mannosyltransferase involved in extending the α1-6-mannan backbone of LM intermediates. Interestingly, NCgl2760 lacks any motifs or homology to other proteins of known function. Attempts to delete the NCgl2760 orthologue in Mycobacterium smegmatis were unsuccessful, consistent with previous studies indicating that the M. tuberculosis orthologue, Rv0227c, is an essential gene. Together, these data suggest that NCgl2760/ Rv0227c plays a critical role in the elongation of the mannan backbone of mycobacterial and corynebacterial LM, further highlighting the complexity of lipoglycan pathways of Corynebacterineae.",
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Identification of a membrane protein required for lipomannan maturation and lipoarabinomannan synthesis in corynebacterineae. / Cashmore, Tamaryn J.; Klatt, Stephan; Yamaryo-Botte, Yoshiki; Brammananth, Rajini; Rainczuk, Arek K.; McConville, Malcolm J; Crellin, Paul K.; Coppel, Ross L.

In: Journal of Biological Chemistry, Vol. 292, No. 12, 24.03.2017, p. 4976-4986.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Identification of a membrane protein required for lipomannan maturation and lipoarabinomannan synthesis in corynebacterineae

AU - Cashmore, Tamaryn J.

AU - Klatt, Stephan

AU - Yamaryo-Botte, Yoshiki

AU - Brammananth, Rajini

AU - Rainczuk, Arek K.

AU - McConville, Malcolm J

AU - Crellin, Paul K.

AU - Coppel, Ross L.

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AB - Mycobacterium tuberculosis and related Corynebacterineae synthesize a family of lipomannans (LM) and lipoarabinomannans (LAM) that are abundant components of the multilaminate cell wall and essential virulence factors in pathogenic species. Here we describe a new membrane protein, highly conserved in all Corynebacterineae, that is required for synthesis of fulllength LM and LAM. Deletion of the Corynebacterium glutamicum NCgl2760 gene resulted in a complete loss of mature LM/LAM and the appearance of a truncated LM (t-LM). Complementation of the mutant with the NCgl2760 gene fully restored LM/LAM synthesis. Structural studies, including monosaccharide analysis, methylation linkage analysis, and mass spectrometry of native LM species, indicated that the ΔNCgl2760 t-LM comprised a series of short LM species (8-27 residues long) containing an α1-6-linked mannose backbone with greatly reduced α1-2-mannose side chains and no arabinose caps. The structure of the ΔNCgl2760 t-LM was similar to that of the t-LM produced by a C. glutamicum mutant lacking the mptA gene, encoding a membrane α1-6-mannosyltransferase involved in extending the α1-6-mannan backbone of LM intermediates. Interestingly, NCgl2760 lacks any motifs or homology to other proteins of known function. Attempts to delete the NCgl2760 orthologue in Mycobacterium smegmatis were unsuccessful, consistent with previous studies indicating that the M. tuberculosis orthologue, Rv0227c, is an essential gene. Together, these data suggest that NCgl2760/ Rv0227c plays a critical role in the elongation of the mannan backbone of mycobacterial and corynebacterial LM, further highlighting the complexity of lipoglycan pathways of Corynebacterineae.

KW - cell wall

KW - glycolipid

KW - lipid synthesis

KW - membrane protein

KW - Mycobacterium tuerculosis

KW - Coryneacterium glutamicum

KW - lipoarabinomannan

KW - lipomannan

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