Identification of a dopamine-binding protein on the membrane of the human platelet

B. Dean, A. J. McAdam, S. Sundram, G. Pavey, L. C. Harrison, D. L. Copolov

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Abstract

The binding of [3H]dopamine to platelet membranes has been examined in an attempt to identify the putative dopamine uptake mechanism of the platelet. [3H]Dopamine has been shown to bind to a 42000 Da glycoprotein in platelet membrane with high affinity (K(d) = 22.6 nM) and binding of [3H]dopamine was competed for by dopamine, molecules with catechol moieties, 5-hydroxytryptamine, GSH and ascorbic acid. Differences in pharmacological profile and molecular mass suggest that [3H]dopamine does not bind to a known receptor, a neuronal-type dopamine transporter or the platelet 5-hydroxytryptamine-uptake site. It is proposed that this novel binding site for dopamine, which has been purified 1000-fold from particulate platelet membrane, is likely to be a component of the dopamine-uptake mechanism of the human platelet.

Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalBiochemical Journal
Volume287
Issue number1
DOIs
Publication statusPublished - 1 Jan 1992
Externally publishedYes

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