Identification of a cluster of genes that directs desferrioxamine biosynthesis in Streptomyces coelicolor M145

Francisco Barona-Gómez, Ursula Wong, Anastassios E. Giannakopulos, Peter J. Derrick, Gregory L. Challis

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183 Citations (Scopus)

Abstract

Desferrioxamines are a structurally related family of tris-hydroxamate siderophores that form strong hexadentate complexes with ferric iron. Desferrioxamine B has been used clinically for the treatment of iron overload in man. We have unambiguously identified desferrioxamine E as the major desferrioxamine siderophore produced by Streptomyces coelicolor M145 and have identified a cluster of four genes (desA-D) that directs desferrioxamine biosynthesis in this model actinomycete. On the basis of comparative sequence analysis of the proteins encoded by these genes, we propose a plausible pathway for desferrioxamine biosynthesis. The desferrioxamine biosynthetic pathway belongs to a new and rapidly emerging family of pathways for siderophore biosynthesis, widely distributed across diverse species of bacteria, which is biochemically distinct from the better known nonribosomal peptide synthetase (NRPS) pathway used in many organisms for siderophore biosynthesis.

Original languageEnglish
Pages (from-to)16282-16283
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number50
DOIs
Publication statusPublished - 22 Dec 2004
Externally publishedYes

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