Identification and molecular characterization of novel and divergent HoBi-like pestiviruses from naturally infected cattle in India

N. Mishra, K. Rajukumar, A. Pateriya, M. Kumar, P. Dubey, S.P. Behera, A. Verma, P. Bhardwaj, D.D. Kulkarni, D. Vijaykrishna, N.D. Reddy

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Abstract

HoBi-like pestiviruses have been sporadically reported from naturally infected cattle in South America, Asia and Europe. While the closely related bovine viral diarrhoea virus 1 (BVDV-1) and BVDV-2 have been reported from cattle in India, the prevalence and diversity of HoBi-like viruses have not yet been studied. Here we report the genetic diversity and molecular characteristics of HoBi-like viruses, through systematic surveillance in cattle (n=1049) from 21 dairy farms across India during 2012-2013. On the basis of real-time RT-PCR, virus isolation and nucleotide sequencing results, of the 20 pestivirus positive cattle, HoBi-like viruses were identified in 19 cattle from four farms in three states and BVDV-1b in one cattle. Phylogenetic analysis of 5'-UTR and Npro region identified the circulation of two lineages of HoBi-like viruses in India, that were distinct to those circulating globally, highlighting the independent evolution of at least three lineages of HoBi-like viruses globally. Antigenic differences were also evident between the two Indian lineages. In addition to revealing that HoBi-like virus may be more widespread in Indian cattle than previously reported, this study shows greater genetic divergence of HoBi-like viruses indicating a need for continued pestivirus surveillance in cattle.

Original languageEnglish
Pages (from-to)239-246
Number of pages8
JournalVeterinary Microbiology
Volume174
Issue number1-2
DOIs
Publication statusPublished - 7 Nov 2014
Externally publishedYes

Keywords

  • 5'-UTR
  • Antigenic characterization
  • Cattle
  • HoBi-like virus
  • Npro
  • Phylogenetic analysis

Cite this

Mishra, N., Rajukumar, K., Pateriya, A., Kumar, M., Dubey, P., Behera, S. P., Verma, A., Bhardwaj, P., Kulkarni, D. D., Vijaykrishna, D., & Reddy, N. D. (2014). Identification and molecular characterization of novel and divergent HoBi-like pestiviruses from naturally infected cattle in India. Veterinary Microbiology, 174(1-2), 239-246. https://doi.org/10.1016/j.vetmic.2014.09.017