Identification and characterisation of a novel conserved outer membrane protein from Neisseria meningitidis

Ian R A Peak, Yogitha Srikhanta, Manuela Dieckelmann, E. Richard Moxon, Michael P. Jennings

Research output: Contribution to journalArticleResearchpeer-review

49 Citations (Scopus)

Abstract

We have identified a homologue of the adhesin AIDA-I of Escherichia coli in Neisseria meningitidis. This gene was designated nhhA (Neisseria hia homologue), as analysis of the complete coding sequence revealed that it is more closely related to the adhesins Hia and Hsf of Haemophilus influenzae. The sequence of nhhA was determined from 10 strains, and found to be highly conserved. Studies of the localisation by Western immunoblot analysis of total cell proteins and outer membrane complex preparations and by immunogold electron microscopy revealed that NhhA is located in the outer membrane. A strain survey showed that nhhA is present in 85/85 strains of N. meningitidis representative of all the major disease-associated serogroups, based on Southern blot analysis. It is expressed in the majority of strains tested by Western immunoblot. Copyright (C) 2000.

Original languageEnglish
Pages (from-to)329-334
Number of pages6
JournalFEMS Immunology and Medical Microbiology
Volume28
Issue number4
DOIs
Publication statusPublished - 1 Aug 2000
Externally publishedYes

Keywords

  • Adhesin
  • High molecular mass
  • Neisseria meningitidis
  • Outer membrane protein

Cite this