@article{1dc38d8c63b34130ad41a85ab5e2749e,
title = "Aquella oligotrophica gen. nov. sp. nov. A new member of the family Neisseriaceae isolated from laboratory tap water",
abstract = "A bacterial strain designated as P08T was isolated from laboratory tap water during a water quality assessment in University of Malaya, Malaysia. The strain was a Gram-negative, rod-shaped, nonmotile, and aerobic bacterium. Complete genome of P08T comprised of a 2,820,660 bp chromosome with a G + C content of 36.43\%. Both 16S rRNA phylogeny and phylogenetic tree inferred from the core gene matrix demonstrated that P08T formed a hitherto unknown subline within the family Neisseriaceae. Ortho average nucleotide identity (OrthoANI) values and the percentage of conserved proteins (POCP) calculated from complete genome sequence indicated low relatedness between P08T and its phylogenetic neighbors. Respiratory quinone analysis revealed Q-8 as the only detectable quinone. The predominant cellular fatty acids were identified as C14:0, iso-C15:0, and summed feature 3 (C16:1 ω7c/C16:1 ω6c). The polar lipids consisted of uncharacterized aminolipid, phosphatidylglycerol, and phosphatidylethanolamine. All aspects of phenotypic and phylogenetic data suggested that strain P08T represents a novel genus within family Neisseriaceae, for which the name Aquella gen. nov. is proposed. The type species of the genus is Aquella oligotrophica sp. nov., and the type strain is P08T (=LMG 29629T =DSM 100970T).",
keywords = "Aquella oligotrophica gen. nov. sp. nov., Neisseriaceae, OrthoANI, POCP, tap water",
author = "Chan, \{Kok Gan\} and See-Too, \{Wah Seng\} and Chua, \{Kah Ooi\} and {\'A}lvaro Peix and Goh, \{Kian Mau\} and Hong, \{Kar Wai\} and Yin, \{Wai Fong\} and Lee, \{Li Sin\}",
note = "Funding Information: University of Malaya–Ministry of Higher Education High Impact Research (UM‐ MOHE HIR) Grant, Grant/Award Number: UM.C/625/1/HIR/MOHE/CHAN/14/1 and H‐ 50001‐A000027; University of Malaya Research Grants, Grant/Award Number: GA001‐2016 and GA002‐2016; Postgraduate Research Grant (PPP), Grant/ Award Number: PG124‐2016A, UTM GUP Grant and 15H50; UTM GUP grant Funding Information: This work was supported by University of Malaya—Ministry of Higher Education High Impact Research Grant (UM‐MOHE HIR Grant UM.C/625/1/HIR/MOHE/CHAN/14/1, Grant No. H‐ 50001‐ A000027) and University of Malaya Research Grants (GA001‐2016, GA002‐2016) awarded to Kok‐Gan Chan and Postgraduate Research Grant (PPP) (Grant No. PG124‐2016A) awarded to Li Sin Lee. Kian Mau Goh thanks UTM GUP grant 15H50. Wah‐Seng See‐Too thanks Bright Sparks Unit of University of Malaya for the scholarship sup‐ port. The authors thank Jian‐Woon Chen from High Impact Research Institute of University of Malaya in providing the GEN III service and Dr. Aidan Parte for his help in defining the Latin etymology of the novel taxon scientific name. Funding Information: This work was supported by University of Malaya—Ministry of Higher Education High Impact Research Grant (UM-MOHE HIR Grant UM.C/625/1/HIR/MOHE/CHAN/14/1, Grant No. H- 50001-A000027) and University of Malaya Research Grants (GA001-2016, GA002-2016) awarded to Kok-Gan Chan and Postgraduate Research Grant (PPP) (Grant No. PG124-2016A) awarded to Li Sin Lee. Kian Mau Goh thanks UTM GUP grant 15H50. Wah-Seng See-Too thanks Bright Sparks Unit of University of Malaya for the scholarship support. The authors thank Jian-Woon Chen from High Impact Research Institute of University of Malaya in providing the GEN III service and Dr. Aidan Parte for his help in defining the Latin etymology of the novel taxon scientific name. Publisher Copyright: {\textcopyright} 2019 The Authors. MicrobiologyOpen published by John Wiley \& Sons Ltd.",
year = "2019",
month = jul,
doi = "10.1002/mbo3.793",
language = "English",
volume = "8",
journal = "MicrobiologyOpen",
issn = "2045-8827",
publisher = "Wiley-Blackwell",
number = "7",
}