Actinomadura graeca sp. nov. A novel producer of the macrocyclic antibiotic zelkovamycin

Francesco Saverio Tarantini, Mara Brunati, Anna Taravella, Lucia Carrano, Francesco Parenti, Kar Wai Hong, Paul Williams, Kok Gan Chan, Stephan Heeb, Weng C. Chan

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

As part of a screening programme for antibiotic-producing bacteria, a novel Actinomadura species was discovered from a soil sample collected in Santorini, Greece. Preliminary 16S rRNA gene sequence comparisons highlighted Actinomadura macra as the most similar characterised species. However, whole-genome sequencing revealed an average nucleotide identity (ANI) value of 89% with A. macra, the highest among related species. Further phenotypic and chemotaxonomic analyses confirmed that the isolate represents a previously uncharacterised species in the genus Actinomadura, for which the name Actinomadura graeca sp. nov. is proposed (type strain 32-07T). The G+C content of A. graeca 32-07 is 72.36%. The cell wall contains DL-diaminopimelic acid, intracellular sugars are glucose, ribose and galactose, the predominant menaquinone is MK-9(H6), the major cellular lipid is phosphatidylinositol and fatty acids consist mainly of hexadecanoic acid. No mycolic acid was detected. Furthermore, A. graeca 32-07 has been confirmed as a novel producer of the non-ribosomal peptide antibiotic zelkovamycin and we report herein a provisional description of the unique biosynthetic gene cluster.

Original languageEnglish
Article numbere0260413
Number of pages23
JournalPLoS ONE
Volume16
Issue number11
DOIs
Publication statusPublished - Nov 2021
Externally publishedYes

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