Hypervirulence and biofilm production in KPC-2-producing klebsiella pneumoniae CG258 isolated in Brazil

Bruna Fuga Araújo, Melina Lorraine Ferreira, Paola Amaral de Campos, Sabrina Royer, Iara Rossi Gonçalves, Deivid William da Fonseca Batistão, Miriam Rodriguez Fernandes, Louise Teixeira Cerdeira, Cristiane Silveira de Brito, Nilton Lincopan, Paulo Pinto Gontijo-Filho, Rosineide Marques Ribas

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12 Citations (Scopus)


In this study, we describe the frequency of virulence genes in Klebsiella pneumoniae carbapenemase-2-producing Klebsiella pneumoniae (KPC-KP), including hypervirulent (hv) and hypermucoviscous (hm) strains by whole-genome sequencing. We also evaluate the capacity for biofilm formation by using phenotypic techniques. The occurrence of several virulence genes (fimABCDEFGHIK, mrkABCDFHJ, ecpA, wabG, entB, ugE, irp1, irp2, traT, iutA and ureADE) and a high frequency of hvhmKPC-KP isolates was found. Most hospital-associated lineages of KPC-KP belong to the international clonal group 258 (CG258). Biofilm formation was a constant feature among 90.9% of KPC-KP strains. This report suggests a close relationship between ST437 and weak biofilm production, given that all weakly biofilm-producing strains belonged to this sequence type. This also supports the dissemination of KPC-KP containing numerous virulence determinants belonging to the biofilm-producing CG258 type in Brazil, including hv and hm strains. These factors allow this pathogen to cause infections, leading to its rapid expansion and persistence in hospital settings.

Original languageEnglish
Pages (from-to)523-528
Number of pages6
JournalJournal of Medical Microbiology
Issue number4
Publication statusPublished - Apr 2018
Externally publishedYes


  • Biofilm
  • Hypervirulence
  • Klebsiella pneumoniae
  • KPC-2
  • WGS

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