Hybrid treatment via MHz acoustic waves and plasma to enhance seed germination in mung bean

We investigate a hybrid treatment - consisting of an atmospheric pressure plasma pretreatment, followed by an MHz surface acoustic waves (SAWs) treatment with either de-ionized (DI) water or plasma activated water (PAW) - on mung beans to accelerate the germination process, as mung bean sprout is one of the important food staples. For the early growth rate (after 320 min), we observe that the hybrid treatment with PAW can lead to approximately 217% higher moisture content for the treated beans when compared with that without hybrid treatment. Additionally, the hybrid-treated beans germinate in around 120 min, while the untreated beans germinate only in around 420 min, that is, 3.5-fold faster for treated beans. This can be attributed to the dominant effect of SAW that accelerates stage 1 water absorption process and the effect of direct plasma and PAW that promote stage 2 metabolism process, leading to the enhancement in stage 3 germination process in early growth rate. For the post growth rate (after 24 h), we observe that the hybrid treatment with DI water can lead to an approximately 44.20% in higher moisture and 71.17% in radicle length when compared with untreated beans. Interestingly, the hybrid treatment with PAW, on the other hand, is observed to have an adverse effect on germination after 24 h, that is, approximately 14.51% lower in moisture content and 43.49% lower in radicle length for the hybrid-treated beans with PAW when compared with that with DI water.