Abstract
We aimed to generate T-cell clones specific for human pre-proinsulin. An HLA DQ8, CD4+ T-cell clone that recognised a 10mer (C65-A9) peptide from pre-proinsulin was isolated. Further analysis revealed that the clone responded neither to recombinant proinsulin nor to re-synthesised C65-A9 peptide. Analysis of the original peptide revealed minor contamination (<0.5%) with an N-terminal Fmoc adduct. This peptide was synthesised and shown to stimulate the clone. Thus, Fmoc-modified peptides, which are common contaminants in synthetic peptides, can stimulate human CD4+ T-cells. This finding has important implications for the use of synthetic peptides in screening and epitope mapping studies and their use as vaccines in humans.
| Original language | English |
|---|---|
| Pages (from-to) | 3638-3646 |
| Number of pages | 9 |
| Journal | Vaccine |
| Volume | 21 |
| Issue number | 25-26 |
| DOIs | |
| Publication status | Published - 1 Jan 2003 |
| Externally published | Yes |
Keywords
- Fmoc
- Peptides
- T lymphocytes
Cite this
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Human T-cells recognise N-terminally Fmoc-modified peptide. / Mannering, Stuart I.; Purcell, Anthony W.; Honeyman, Margo C.; McCluskey, James; Harrison, Leonard C.
In: Vaccine, Vol. 21, No. 25-26, 01.01.2003, p. 3638-3646.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Human T-cells recognise N-terminally Fmoc-modified peptide
AU - Mannering, Stuart I.
AU - Purcell, Anthony W.
AU - Honeyman, Margo C.
AU - McCluskey, James
AU - Harrison, Leonard C.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - We aimed to generate T-cell clones specific for human pre-proinsulin. An HLA DQ8, CD4+ T-cell clone that recognised a 10mer (C65-A9) peptide from pre-proinsulin was isolated. Further analysis revealed that the clone responded neither to recombinant proinsulin nor to re-synthesised C65-A9 peptide. Analysis of the original peptide revealed minor contamination (<0.5%) with an N-terminal Fmoc adduct. This peptide was synthesised and shown to stimulate the clone. Thus, Fmoc-modified peptides, which are common contaminants in synthetic peptides, can stimulate human CD4+ T-cells. This finding has important implications for the use of synthetic peptides in screening and epitope mapping studies and their use as vaccines in humans.
AB - We aimed to generate T-cell clones specific for human pre-proinsulin. An HLA DQ8, CD4+ T-cell clone that recognised a 10mer (C65-A9) peptide from pre-proinsulin was isolated. Further analysis revealed that the clone responded neither to recombinant proinsulin nor to re-synthesised C65-A9 peptide. Analysis of the original peptide revealed minor contamination (<0.5%) with an N-terminal Fmoc adduct. This peptide was synthesised and shown to stimulate the clone. Thus, Fmoc-modified peptides, which are common contaminants in synthetic peptides, can stimulate human CD4+ T-cells. This finding has important implications for the use of synthetic peptides in screening and epitope mapping studies and their use as vaccines in humans.
KW - Fmoc
KW - Peptides
KW - T lymphocytes
UR - http://www.scopus.com/inward/record.url?scp=0242380659&partnerID=8YFLogxK
U2 - 10.1016/S0264-410X(03)00402-X
DO - 10.1016/S0264-410X(03)00402-X
M3 - Article
VL - 21
SP - 3638
EP - 3646
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 25-26
ER -