Human T-cells recognise N-terminally Fmoc-modified peptide

Stuart I. Mannering, Anthony W. Purcell, Margo C. Honeyman, James McCluskey, Leonard C. Harrison

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13 Citations (Scopus)

Abstract

We aimed to generate T-cell clones specific for human pre-proinsulin. An HLA DQ8, CD4+ T-cell clone that recognised a 10mer (C65-A9) peptide from pre-proinsulin was isolated. Further analysis revealed that the clone responded neither to recombinant proinsulin nor to re-synthesised C65-A9 peptide. Analysis of the original peptide revealed minor contamination (<0.5%) with an N-terminal Fmoc adduct. This peptide was synthesised and shown to stimulate the clone. Thus, Fmoc-modified peptides, which are common contaminants in synthetic peptides, can stimulate human CD4+ T-cells. This finding has important implications for the use of synthetic peptides in screening and epitope mapping studies and their use as vaccines in humans.

Original languageEnglish
Pages (from-to)3638-3646
Number of pages9
JournalVaccine
Volume21
Issue number25-26
DOIs
Publication statusPublished - 1 Jan 2003
Externally publishedYes

Keywords

  • Fmoc
  • Peptides
  • T lymphocytes

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