Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells

Mio Nakanishi, Ryan R. Mitchell, Yannick D. Benoit, Luca Orlando, Jennifer C. Reid, Kenichi Shimada, Kathryn C. Davidson, Zoya Shapovalova, Tony J. Collins, Andras Nagy, Mickie Bhatia

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The assembly of organized colonies is the earliest manifestation in the derivation or induction of pluripotency in vitro. However, the necessity and origin of this assemblance is unknown. Here, we identify human pluripotent founder cells (hPFCs) that initiate, as well as preserve and establish, pluripotent stem cell (PSC) cultures. PFCs are marked by N-cadherin expression (NCAD + ) and reside exclusively at the colony boundary of primate PSCs. As demonstrated by functional analysis, hPFCs harbor the clonogenic capacity of PSC cultures and emerge prior to commitment events or phenotypes associated with pluripotent reprogramming. Comparative single-cell analysis with pre- and post-implantation primate embryos revealed hPFCs share hallmark properties with primitive endoderm (PrE) and can be regulated by non-canonical Wnt signaling. Uniquely informed by primate embryo organization in vivo, our study defines a subset of founder cells critical to the establishment pluripotent state. Human pluripotent founder cells within stem cell cultures share hallmark properties with primitive endoderm and reside exclusively at colony boundaries.

Original languageEnglish
Pages (from-to)910-924
Number of pages15
JournalCell
Volume177
Issue number4
DOIs
Publication statusPublished - 2 May 2019

Keywords

  • cell fate
  • founder
  • human development
  • non-canonical Wnt signaling
  • pluripotency
  • pluripotent state
  • primate
  • reprogramming
  • self-renewal
  • single cell RNA-seq

Cite this

Nakanishi, M., Mitchell, R. R., Benoit, Y. D., Orlando, L., Reid, J. C., Shimada, K., ... Bhatia, M. (2019). Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells. Cell, 177(4), 910-924. https://doi.org/10.1016/j.cell.2019.03.013
Nakanishi, Mio ; Mitchell, Ryan R. ; Benoit, Yannick D. ; Orlando, Luca ; Reid, Jennifer C. ; Shimada, Kenichi ; Davidson, Kathryn C. ; Shapovalova, Zoya ; Collins, Tony J. ; Nagy, Andras ; Bhatia, Mickie. / Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells. In: Cell. 2019 ; Vol. 177, No. 4. pp. 910-924.
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Nakanishi, M, Mitchell, RR, Benoit, YD, Orlando, L, Reid, JC, Shimada, K, Davidson, KC, Shapovalova, Z, Collins, TJ, Nagy, A & Bhatia, M 2019, 'Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells' Cell, vol. 177, no. 4, pp. 910-924. https://doi.org/10.1016/j.cell.2019.03.013

Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells. / Nakanishi, Mio; Mitchell, Ryan R.; Benoit, Yannick D.; Orlando, Luca; Reid, Jennifer C.; Shimada, Kenichi; Davidson, Kathryn C.; Shapovalova, Zoya; Collins, Tony J.; Nagy, Andras; Bhatia, Mickie.

In: Cell, Vol. 177, No. 4, 02.05.2019, p. 910-924.

Research output: Contribution to journalArticleResearchpeer-review

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Nakanishi M, Mitchell RR, Benoit YD, Orlando L, Reid JC, Shimada K et al. Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells. Cell. 2019 May 2;177(4):910-924. https://doi.org/10.1016/j.cell.2019.03.013