Human KLF17 is a new member of the Sp/KLF family of transcription factors

Jane Van Vliet, Linda A. Crofts, Kate G.R. Quinlan, Robert Czolij, Andrew C. Perkins, Merlin Crossley

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Abstract

The Sp/KLF transcription factors perform a variety of biological functions, but are related in that they bind GC-box and CACCC-box sequences in DNA via a highly conserved DNA-binding domain. A database homology search, using the zinc finger DNA-binding domain characteristic of the family, has identified human KLF17 as a new family member that is most closely related to KLFs 1-8 and 12. KLF17 appears to be the human orthologue of the previously reported mouse gene, zinc finger protein 393 (Zfp393), although it has diverged significantly. The DNA-binding domain is the most conserved region, suggesting that both the murine and the human forms recognize the same binding sites in DNA and may retain similar functions. We show that human KLF17 can bind G/C-rich sites via its zinc fingers and is able to activate transcription from CACCC-box elements. This is the first report of the DNA-binding characteristics and transactivation activity of human KLF17, which, together with the homology it displays to other KLF proteins, put it in the Sp/KLF family.

Original languageEnglish
Pages (from-to)474-482
Number of pages9
JournalGenomics
Volume87
Issue number4
DOIs
Publication statusPublished - Apr 2006
Externally publishedYes

Keywords

  • CACCC box
  • GC box
  • Krüppel-like factor
  • Zinc finger protein 393
  • ZNF393

Cite this

Van Vliet, J., Crofts, L. A., Quinlan, K. G. R., Czolij, R., Perkins, A. C., & Crossley, M. (2006). Human KLF17 is a new member of the Sp/KLF family of transcription factors. Genomics, 87(4), 474-482. https://doi.org/10.1016/j.ygeno.2005.12.011