Human growth hormone releasing factor (hGRF) modulates calcium currents in human growth hormone secreting adenoma cells

Chen Chen; Jin Zhang; Peter McNeill; Michael Pullar; James T. Cummins; Iain J. Clarke

doi:10.1016/0006-8993(93)90389-5

Human growth hormone releasing factor (hGRF) modulates calcium currents in human growth hormone secreting adenoma cells

Chen Chen, Jin Zhang, Peter McNeill, Michael Pullar, James T. Cummins, Iain J. Clarke

Research output: Contribution to journal › Article › Research › peer-review

19 Citations (Scopus)

Abstract

Electrophysiology of human growth hormone secreting tumour cells and its modification by hGRF has been studied using on-cell and Nystatin-perforated whole-cell recording configurations. Local application of hGRF (10 nM) produced an increase in the frequency of action potentials. Ca²⁺ currents were isolated by a ramp depolarizing pulse from -120 mV to +60 mV in the presence of tetrodotoxin (1 μM). Human GRF increased the Ca²⁺ currents which could be blocked by Ni⁺ (300 μM). We conclude that an increase in Ca²⁺ current is integral to the action of hGRF on these cells.

Original language	English
Pages (from-to)	345-348
Number of pages	4
Journal	Brain Research
Volume	604
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(93)90389-5
Publication status	Published - 26 Feb 1993
Externally published	Yes

Keywords

Acromegaly
Ca current
Electrophysiology
Growth hormone
Patch-clamp
Somatotroph

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10.1016/0006-8993(93)90389-5

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abstract = "Electrophysiology of human growth hormone secreting tumour cells and its modification by hGRF has been studied using on-cell and Nystatin-perforated whole-cell recording configurations. Local application of hGRF (10 nM) produced an increase in the frequency of action potentials. Ca2+ currents were isolated by a ramp depolarizing pulse from -120 mV to +60 mV in the presence of tetrodotoxin (1 μM). Human GRF increased the Ca2+ currents which could be blocked by Ni+ (300 μM). We conclude that an increase in Ca2+ current is integral to the action of hGRF on these cells.",

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AU - Chen, Chen

AU - Zhang, Jin

AU - McNeill, Peter

AU - Pullar, Michael

AU - Cummins, James T.

AU - Clarke, Iain J.

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AB - Electrophysiology of human growth hormone secreting tumour cells and its modification by hGRF has been studied using on-cell and Nystatin-perforated whole-cell recording configurations. Local application of hGRF (10 nM) produced an increase in the frequency of action potentials. Ca2+ currents were isolated by a ramp depolarizing pulse from -120 mV to +60 mV in the presence of tetrodotoxin (1 μM). Human GRF increased the Ca2+ currents which could be blocked by Ni+ (300 μM). We conclude that an increase in Ca2+ current is integral to the action of hGRF on these cells.

KW - Acromegaly

KW - Ca current

KW - Electrophysiology

KW - Growth hormone

KW - Patch-clamp

KW - Somatotroph

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Human growth hormone releasing factor (hGRF) modulates calcium currents in human growth hormone secreting adenoma cells

Abstract

Keywords

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