Human growth hormone releasing factor (hGRF) modulates calcium currents in human growth hormone secreting adenoma cells

Chen Chen, Jin Zhang, Peter McNeill, Michael Pullar, James T. Cummins, Iain J. Clarke

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19 Citations (Scopus)


Electrophysiology of human growth hormone secreting tumour cells and its modification by hGRF has been studied using on-cell and Nystatin-perforated whole-cell recording configurations. Local application of hGRF (10 nM) produced an increase in the frequency of action potentials. Ca2+ currents were isolated by a ramp depolarizing pulse from -120 mV to +60 mV in the presence of tetrodotoxin (1 μM). Human GRF increased the Ca2+ currents which could be blocked by Ni+ (300 μM). We conclude that an increase in Ca2+ current is integral to the action of hGRF on these cells.

Original languageEnglish
Pages (from-to)345-348
Number of pages4
JournalBrain Research
Issue number1-2
Publication statusPublished - 26 Feb 1993
Externally publishedYes


  • Acromegaly
  • Ca current
  • Electrophysiology
  • Growth hormone
  • Patch-clamp
  • Somatotroph

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