Human cytochrome P450's are pro-oxidants in iron/ascorbate-initiated microsomal lipid peroxidation

Nigel Lambert, Stephen J. Chambers, Geoffrey W. Plumb, Gary Williamson

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Abstract

We have examined the effect of human cytochrome P450's (1A1, 1A2, 3A4, 2A6, 2B6, 2D6, 2E1) on ascorbate/ iron-induced lipid peroxidation. Using microsomes prepared from human lymphoblastic cells enriched in recombinant cytochrome P450 isoenzymes, we have shown that the degree of peroxidation is a function of the amount of P450 present rather than the presence of any specific isoenzyme. Incorporated P450 increased the amount of peroxidation products by up to 2.1-fold compared to the control microsomes with no P450. It is therefore concluded that cytochrome P450's play a significant role in ascorbate/iron peroxidation.

Original languageEnglish
Pages (from-to)177-185
Number of pages9
JournalFree Radical Research
Volume24
Issue number3
DOIs
Publication statusPublished - 1 Jan 1996
Externally publishedYes

Keywords

  • Cultured cells
  • Cytochrome P
  • Lipid peroxidation
  • Microsomes

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