Human chorionic gonadotrophin-induced suppression of serum inhibin involves reduction in ovarian inhibin α-subunit messenger RNA levels in the pregnant mare serum gonadotrophin-primed female rat

Susan R. Davis, Bronwyn Matheson, Henry Burger, Zygmunt Krozowski

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

We have investigated the effect of administration of human chorionic gonadotrophin (hCG) to immature female rats pretreated with pregnant mare serum gonadotrophin (PMSG) on ovarian inhibin α-subunit mRNA serum inhibin and circulating gonadotrophin levels. PMSG stimulation alone caused a 5-fold increase in relative inhibin a-subunit mRNA levels and a 12-fold increase in serum inhibin by 48 h. However injection of hCG at 40 h suppressed PMSG-stimulated ovarian inhibin a-subunit mRNA and serum inhibin to levels at 48 h not statistically significantly different from controls. Serum follicle-stimulating hormone (FSH) fell after PMSG treatment but rose after combined PMSG-hCG treatment. Serum luteinizing hormone (LH) was unchanged after PMSG alone but also rose after combined PMSG and hCG therapy. In conclusion hCG suppresses ovarian inhibin synthesis in PMSG-stimulated immature female rats with preservation of the reciprocal relationship between inhibin and FSH. This immature female rat model therefore provides further insight into the effects of LH or hCG on granulosa cell inhibin production just prior to ovulation.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalMolecular and Cellular Endocrinology
Volume61
Issue number1
DOIs
Publication statusPublished - 1989

Keywords

  • Gene expression
  • Human chorionic gonadotropin
  • Inhibin
  • Pregnant mare serum gonadotropin

Cite this