Human blastocyst secreted microRNA regulate endometrial epithelial cell adhesion

Carly Cuman, Michelle Leigh Van Sinderen, Michael Paul Marie Gantier, Katarzyna Eliza (Kate) Rainczuk, Kelli Louise Sorby, Luk Rombauts, Tiki Osianlis, Evdokia Dimitriadis

Research output: Contribution to journalArticleResearchpeer-review

31 Citations (Scopus)

Abstract

Successful embryo implantation requires synchronous development and communication between the blastocyst and the endometrium, however the mechanisms of communication in humans are virtually unknown. Recent studies have revealed that microRNAs (miRs) are present in bodily fluids and secreted by cells in culture. We have identified that human blastocysts differentially secrete miRs in a pattern associated with their implantation outcome. miR-661 was the most highly expressed miR in blastocyst culture media (BCM) from blastocysts that failed to implant (non-implanted) compared to blastocysts that implanted (implanted). Our results indicate a possible role for Argonaute 1 in the transport of miR-661 in non-implanted BCM and taken up by primary human endometrial epithelial cells (HEECs). miR-661 uptake by HEEC reduced trophoblast cell line spheroid attachment to HEEC via PVRL1. Our results suggest that human blastocysts alter the endometrial epithelial adhesion, the initiating event of implantation, via the secretion of miR, abnormalities in which result in implantation failure.
Original languageEnglish
Pages (from-to)1528 - 1535
Number of pages8
JournalEBioMedicine
Volume2
Issue number10
DOIs
Publication statusPublished - 2015

Cite this

Cuman, Carly ; Van Sinderen, Michelle Leigh ; Gantier, Michael Paul Marie ; Rainczuk, Katarzyna Eliza (Kate) ; Sorby, Kelli Louise ; Rombauts, Luk ; Osianlis, Tiki ; Dimitriadis, Evdokia. / Human blastocyst secreted microRNA regulate endometrial epithelial cell adhesion. In: EBioMedicine. 2015 ; Vol. 2, No. 10. pp. 1528 - 1535.
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abstract = "Successful embryo implantation requires synchronous development and communication between the blastocyst and the endometrium, however the mechanisms of communication in humans are virtually unknown. Recent studies have revealed that microRNAs (miRs) are present in bodily fluids and secreted by cells in culture. We have identified that human blastocysts differentially secrete miRs in a pattern associated with their implantation outcome. miR-661 was the most highly expressed miR in blastocyst culture media (BCM) from blastocysts that failed to implant (non-implanted) compared to blastocysts that implanted (implanted). Our results indicate a possible role for Argonaute 1 in the transport of miR-661 in non-implanted BCM and taken up by primary human endometrial epithelial cells (HEECs). miR-661 uptake by HEEC reduced trophoblast cell line spheroid attachment to HEEC via PVRL1. Our results suggest that human blastocysts alter the endometrial epithelial adhesion, the initiating event of implantation, via the secretion of miR, abnormalities in which result in implantation failure.",
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Human blastocyst secreted microRNA regulate endometrial epithelial cell adhesion. / Cuman, Carly; Van Sinderen, Michelle Leigh; Gantier, Michael Paul Marie; Rainczuk, Katarzyna Eliza (Kate); Sorby, Kelli Louise; Rombauts, Luk; Osianlis, Tiki; Dimitriadis, Evdokia.

In: EBioMedicine, Vol. 2, No. 10, 2015, p. 1528 - 1535.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Human blastocyst secreted microRNA regulate endometrial epithelial cell adhesion

AU - Cuman, Carly

AU - Van Sinderen, Michelle Leigh

AU - Gantier, Michael Paul Marie

AU - Rainczuk, Katarzyna Eliza (Kate)

AU - Sorby, Kelli Louise

AU - Rombauts, Luk

AU - Osianlis, Tiki

AU - Dimitriadis, Evdokia

PY - 2015

Y1 - 2015

N2 - Successful embryo implantation requires synchronous development and communication between the blastocyst and the endometrium, however the mechanisms of communication in humans are virtually unknown. Recent studies have revealed that microRNAs (miRs) are present in bodily fluids and secreted by cells in culture. We have identified that human blastocysts differentially secrete miRs in a pattern associated with their implantation outcome. miR-661 was the most highly expressed miR in blastocyst culture media (BCM) from blastocysts that failed to implant (non-implanted) compared to blastocysts that implanted (implanted). Our results indicate a possible role for Argonaute 1 in the transport of miR-661 in non-implanted BCM and taken up by primary human endometrial epithelial cells (HEECs). miR-661 uptake by HEEC reduced trophoblast cell line spheroid attachment to HEEC via PVRL1. Our results suggest that human blastocysts alter the endometrial epithelial adhesion, the initiating event of implantation, via the secretion of miR, abnormalities in which result in implantation failure.

AB - Successful embryo implantation requires synchronous development and communication between the blastocyst and the endometrium, however the mechanisms of communication in humans are virtually unknown. Recent studies have revealed that microRNAs (miRs) are present in bodily fluids and secreted by cells in culture. We have identified that human blastocysts differentially secrete miRs in a pattern associated with their implantation outcome. miR-661 was the most highly expressed miR in blastocyst culture media (BCM) from blastocysts that failed to implant (non-implanted) compared to blastocysts that implanted (implanted). Our results indicate a possible role for Argonaute 1 in the transport of miR-661 in non-implanted BCM and taken up by primary human endometrial epithelial cells (HEECs). miR-661 uptake by HEEC reduced trophoblast cell line spheroid attachment to HEEC via PVRL1. Our results suggest that human blastocysts alter the endometrial epithelial adhesion, the initiating event of implantation, via the secretion of miR, abnormalities in which result in implantation failure.

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