Human amnion epithelial cells mediate lung repair by directly modulating macrophage recruitment and polarization

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Human amnion epithelial cells (hAECs) have been shown to modulate inflammation and restore normal lung structure and respiratory function following bleomycin challenge in immune-competent mice. These effects are exerted despite a lack of significant engraftment of hAECs, suggesting that immunomodulatory effect mechanisms are at play. In this study, using the bleomycin model of injury, we explored the interactions between hAECs and macrophages. We administered 4 million hAECs intraperitoneally to C57Bl6 mice 24 h following a bleomycin challenge. Using FACS analysis and qPCR, we showed that hAEC administration significantly reduced macrophage infiltration into the lungs and that the majority of the pulmonary macrophages were of the M2 phenotype. Using bone marrow-derived macrophages, we then showed that hAEC-conditioned media could alter macrophage polarization, migration, and phagocytosis, without affecting macrophage survival or proliferation in vitro. This study provides the first evidence that hAECs directly influence macrophage behavior in a proreparative manner and suggests that hAECs are able to mediate these effects independently of other immune cell types.
Original languageEnglish
Pages (from-to)319 - 328
Number of pages10
JournalCell Transplantation: the regenerative medicine journal
Volume23
Issue number3
DOIs
Publication statusPublished - 2014

Cite this

@article{8e02b1f45e5546c4a2e31cf964d547f1,
title = "Human amnion epithelial cells mediate lung repair by directly modulating macrophage recruitment and polarization",
abstract = "Human amnion epithelial cells (hAECs) have been shown to modulate inflammation and restore normal lung structure and respiratory function following bleomycin challenge in immune-competent mice. These effects are exerted despite a lack of significant engraftment of hAECs, suggesting that immunomodulatory effect mechanisms are at play. In this study, using the bleomycin model of injury, we explored the interactions between hAECs and macrophages. We administered 4 million hAECs intraperitoneally to C57Bl6 mice 24 h following a bleomycin challenge. Using FACS analysis and qPCR, we showed that hAEC administration significantly reduced macrophage infiltration into the lungs and that the majority of the pulmonary macrophages were of the M2 phenotype. Using bone marrow-derived macrophages, we then showed that hAEC-conditioned media could alter macrophage polarization, migration, and phagocytosis, without affecting macrophage survival or proliferation in vitro. This study provides the first evidence that hAECs directly influence macrophage behavior in a proreparative manner and suggests that hAECs are able to mediate these effects independently of other immune cell types.",
author = "Jean Tan and Chan, {Siow Teng} and Wallace, {Euan Morrison} and Lim, {Rebecca Seok Wai}",
year = "2014",
doi = "10.3727/096368912X661409",
language = "English",
volume = "23",
pages = "319 -- 328",
journal = "Cell Transplantation: the regenerative medicine journal",
issn = "0963-6897",
publisher = "Cognizant Communication Corporation",
number = "3",

}

TY - JOUR

T1 - Human amnion epithelial cells mediate lung repair by directly modulating macrophage recruitment and polarization

AU - Tan, Jean

AU - Chan, Siow Teng

AU - Wallace, Euan Morrison

AU - Lim, Rebecca Seok Wai

PY - 2014

Y1 - 2014

N2 - Human amnion epithelial cells (hAECs) have been shown to modulate inflammation and restore normal lung structure and respiratory function following bleomycin challenge in immune-competent mice. These effects are exerted despite a lack of significant engraftment of hAECs, suggesting that immunomodulatory effect mechanisms are at play. In this study, using the bleomycin model of injury, we explored the interactions between hAECs and macrophages. We administered 4 million hAECs intraperitoneally to C57Bl6 mice 24 h following a bleomycin challenge. Using FACS analysis and qPCR, we showed that hAEC administration significantly reduced macrophage infiltration into the lungs and that the majority of the pulmonary macrophages were of the M2 phenotype. Using bone marrow-derived macrophages, we then showed that hAEC-conditioned media could alter macrophage polarization, migration, and phagocytosis, without affecting macrophage survival or proliferation in vitro. This study provides the first evidence that hAECs directly influence macrophage behavior in a proreparative manner and suggests that hAECs are able to mediate these effects independently of other immune cell types.

AB - Human amnion epithelial cells (hAECs) have been shown to modulate inflammation and restore normal lung structure and respiratory function following bleomycin challenge in immune-competent mice. These effects are exerted despite a lack of significant engraftment of hAECs, suggesting that immunomodulatory effect mechanisms are at play. In this study, using the bleomycin model of injury, we explored the interactions between hAECs and macrophages. We administered 4 million hAECs intraperitoneally to C57Bl6 mice 24 h following a bleomycin challenge. Using FACS analysis and qPCR, we showed that hAEC administration significantly reduced macrophage infiltration into the lungs and that the majority of the pulmonary macrophages were of the M2 phenotype. Using bone marrow-derived macrophages, we then showed that hAEC-conditioned media could alter macrophage polarization, migration, and phagocytosis, without affecting macrophage survival or proliferation in vitro. This study provides the first evidence that hAECs directly influence macrophage behavior in a proreparative manner and suggests that hAECs are able to mediate these effects independently of other immune cell types.

UR - http://dx.doi.org/10.3727/096368912X661409

U2 - 10.3727/096368912X661409

DO - 10.3727/096368912X661409

M3 - Article

VL - 23

SP - 319

EP - 328

JO - Cell Transplantation: the regenerative medicine journal

JF - Cell Transplantation: the regenerative medicine journal

SN - 0963-6897

IS - 3

ER -