Hu ly-m3—a human leukocyte antigen

Hilary A. Vaughan, Christopher H. Thompson, Rosemary L. Sparrow, Ian F.C. McKenzie

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Abstract

A monoclonal antibody, 5–4.8, was produced against human peripheral blood lymphocytes and it appears to be leukocyte-specific in that it reacts with a common determinant (called Hu Ly-m3) present on the peripheral blood T, B and null lymphocytes of 40 individuals. The antibody also reacts with thymocytes, spleen cells, and bone marrow cells (30%) and weakly with granulocytes and platelets―but not with heart, liver, or kidney. Affinity to lentil-lectin and molecular weight analysis demonstrated that Hu Ly-m3 is a glycoprotein consisting of a single chain of 47, 000 daltons which is not HLA because it is not present on all cells; because it is present on the surface of the phenotypically HLA Daudi cell line; and because soluble HLA antigens did not inhibit the binding of the 5–4.8 antibody.

Original languageEnglish
Pages (from-to)446-450
Number of pages5
JournalTransplantation
Volume36
Issue number4
DOIs
Publication statusPublished - Oct 1983
Externally publishedYes

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