HPLC of peptides and proteins.

R. I. Boysen, M. T. Hearn

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Abstract

High-performance liquid chromatography (HPLC) is an essential tool for the purification and characterization of biomacromolecules. This unit presents a thorough discussion of the eight types of HPLC currently used, highlighting equipment and start-up procedures, recommendations for running each type of experiment, and theoretical considerations for the separation of peptides and proteins. This is an excellent primer for HPLC users.

Original languageEnglish
JournalCurrent Protocols in Protein Science
Volume23
Issue number1
Publication statusPublished - May 2001

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