Abstract
High-performance liquid chromatography (HPLC) is an essential tool for the purification and characterization of biomacromolecules. This unit presents a thorough discussion of the eight types of HPLC currently used, highlighting equipment and start-up procedures, recommendations for running each type of experiment, and theoretical considerations for the separation of peptides and proteins. This is an excellent primer for HPLC users.
| Original language | English |
|---|---|
| Journal | Current Protocols in Protein Science |
| Volume | 23 |
| Issue number | 1 |
| Publication status | Published - May 2001 |