HLA-DR, DQ and anti-GAD antibodies in first degree relatives of type I diabetes mellitus

M. Serrano-Ríos, M. D. Gutierrez-López, F. Pérez-Bravo, M. T. Martínez, J. Antona, M. Rowley, I. Mackay, P. Zimmet

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Abstract

The differential antibody response to glutamic acid decarboxylase (anti-GAD) and to islet cell cytoplasm (ICA) according to HLA-DR and DQ genotypes were examined in 28 Spanish patients with Type I diabetes mellitus (11.1 ± 10.4 year diabetes duration) and their 41 first degree non-diabetic relatives. Anti-GAD was detected by radioimmunoprecipitation and ICA by indirect immunofluorescence and HLA-DR/DQ alleles were assigned by PCR and sequence specific oligonucleotide probes. The frequency in patients of positivity for ICA was 7.1% and of anti-GAD+ 64.3% and in relatives, the frequency of ICA+ was 4.9%, and anti-GAD+ 9.8%. Concurrent positivity for ICA and anti-GAD existed in only one patient, and in none of the relatives. We confirm for a Spanish population the high frequency of risk genotypes for Type I, involving DR3, DR4 and DQB1*0302 (DQ8), which were present in 26 of 28 (93%) patients and 32 of 41 (78%) relatives. The most frequent genotypes were DR3/DQB1*0201/DQA1*0501-DR4/DQB1*0302/DQA1*0301 (9 patients, 32%; 6 relatives, 15%), DR3/DQB1*0201/DQA1*0501-DR3/DQB1*0201/DQA1*0501 (5 patients, 18%; 7 relatives, 17%) and DR3/DQB1*0201/DQA1*0501DR1/DQB1*0501/DQA1*0l01 (5 patients, 18%; 1 relative, 2%). Positivity for anti-GAD or for ICA did not correlate with gender, or age at onset or duration of DM. The distribution of high risk HLA genotypes were similar regardless of the anti-GAD or anti-ICA status either in patients or in their relatives.

Original languageEnglish
Pages (from-to)S133-S139
Number of pages7
JournalDiabetes Research and Clinical Practice
Volume34
Issue numberSUPPL.
DOIs
Publication statusPublished - Oct 1996

Keywords

  • glutamic acid decarboxylase
  • HLA/DR/DQ
  • islet cell cytoplasm
  • Spanish families
  • type I (insulin-dependent) diabetes mellitus

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