HIV taken by STORM: Super-resolution fluorescence microscopy of a viral infection

Candida da Fonseca Pereira, Jeremie Rossy, Dylan M Owen, Johnson Mak, Katharina Gaus

Research output: Contribution to journalArticleResearchpeer-review

30 Citations (Scopus)

Abstract

The visualization of viral proteins has been hindered by the resolution limit of conventional fluorescent microscopes, as the dimension of any single fluorescent signal is often greater than most virion particles. Super-resolution microscopy has the potential to unveil the distribution of proteins at the resolution approaching electron microscopy without relying on morphological features of existing features of the biological specimen. RESULTS: Using direct stochastic optical reconstruction microscopy (dSTORM) to achieve a lateral resolution of 15-20 nm, we quantified the 2-D molecular distribution of the major structural proteins of the infectious human immunodeficiency virus type 1 (HIV-1) before and after infection of lymphoid cells. We determined that the HIV-1 matrix and capsid proteins undergo restructuring soon after HIV-1 infection. CONCLUSIONS: This study provides the proof-of-concept for the use of dSTORM to visualize the changes in the molecular distribution of viral proteins during an infection.
Original languageEnglish
Pages (from-to)1 - 6
Number of pages6
JournalVirology Journal
Volume9
Issue numberArt. No.: 84
DOIs
Publication statusPublished - 2012

Cite this

Pereira, C. D. F., Rossy, J., Owen, D. M., Mak, J., & Gaus, K. (2012). HIV taken by STORM: Super-resolution fluorescence microscopy of a viral infection. Virology Journal, 9(Art. No.: 84), 1 - 6. https://doi.org/10.1186/1743-422X-9-84
Pereira, Candida da Fonseca ; Rossy, Jeremie ; Owen, Dylan M ; Mak, Johnson ; Gaus, Katharina. / HIV taken by STORM: Super-resolution fluorescence microscopy of a viral infection. In: Virology Journal. 2012 ; Vol. 9, No. Art. No.: 84. pp. 1 - 6.
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Pereira, CDF, Rossy, J, Owen, DM, Mak, J & Gaus, K 2012, 'HIV taken by STORM: Super-resolution fluorescence microscopy of a viral infection', Virology Journal, vol. 9, no. Art. No.: 84, pp. 1 - 6. https://doi.org/10.1186/1743-422X-9-84

HIV taken by STORM: Super-resolution fluorescence microscopy of a viral infection. / Pereira, Candida da Fonseca; Rossy, Jeremie; Owen, Dylan M; Mak, Johnson; Gaus, Katharina.

In: Virology Journal, Vol. 9, No. Art. No.: 84, 2012, p. 1 - 6.

Research output: Contribution to journalArticleResearchpeer-review

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AB - The visualization of viral proteins has been hindered by the resolution limit of conventional fluorescent microscopes, as the dimension of any single fluorescent signal is often greater than most virion particles. Super-resolution microscopy has the potential to unveil the distribution of proteins at the resolution approaching electron microscopy without relying on morphological features of existing features of the biological specimen. RESULTS: Using direct stochastic optical reconstruction microscopy (dSTORM) to achieve a lateral resolution of 15-20 nm, we quantified the 2-D molecular distribution of the major structural proteins of the infectious human immunodeficiency virus type 1 (HIV-1) before and after infection of lymphoid cells. We determined that the HIV-1 matrix and capsid proteins undergo restructuring soon after HIV-1 infection. CONCLUSIONS: This study provides the proof-of-concept for the use of dSTORM to visualize the changes in the molecular distribution of viral proteins during an infection.

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