HIV-specific antibody-dependent phagocytosis matures during HIV infection

Fernanda Ana-Sosa-Batiz, Angus Johnston, Haiyin Liu, Robert John Center, Supachai Rerks-Ngarm, Punnee Pitisuttithum, Sorachai Nitayaphan, Jaranit Kaewkungwal, Jerome H Kim, Nelson L Michael, Anthony D Kelleher, Ivan Stratov, Stephen J Kent, Marit Kramski

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24 Citations (Scopus)

Abstract

Antibody-dependent phagocytosis (ADP) is a potentially important immune mechanism to clear HIV. How HIV-specific ADP responses mature during HIV infection or in response to vaccinations administered, including the partially successful RV144 HIV vaccine, is not known. We established a modified ADP assay to measure internalisation of HIV antibody (Ab)-opsonised targets using a specific hybridisation internalisation probe. Labelled beads were coated with both biotinylated HIV gp140 envelope protein and a fluorescent internalisation probe, opsonised with Abs and incubated with a monocytic cell line. The fluorescence derived from the fluorescent internalisation probe on surface-bound beads, but not from internalised beads, was quenched by the addition of a complementary quencher probe. HIV Env-specific ADP was measured in 31 subjects during primary infection and early chronic HIV infection. Although ADP responses were present early during HIV infection, a significant increase in ADP responses in all 31 subjects studied was detected (P
Original languageEnglish
Pages (from-to)679 - 687
Number of pages9
JournalImmunology and Cell Biology
Volume92
Issue number8
DOIs
Publication statusPublished - 2014

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