HIV reverse transcriptase activity assay: A feasible surrogate for HIV viral load measurement in China

Dedong Huang, Yan Zhuang, Song Zhai, Yang Song, Qingquan Liu, Shuguang Zhao, Shaoyang Wang, Xinhong Li, Wenzhen Kang, Vicki Greengrass, Megan Plate, Suzanne M. Crowe, Yongtao Sun

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

The quantitation of HIV viral load using an assay that measures the activity of reverse transcriptase (RT) may provide an alternative strategy for the monitoring of HIV viral load within resource-limited areas in China. Plasma viral load analyses of 215 samples from 87 patients infected with HIV were detected using the RT activity assay (ExaVir Load versions 2 and 3; Cavidi, Uppsala, Sweden) and RT polymerase chain reaction (PCR) (COBAS TaqMan 48, Amplink version 3.2; Roche Molecular Systems, Branchburg, NJ). The RT activity assay versions 3 (RT3) and 2 (RT2) could detect 95.3% and 86.9% of samples with measurable RNA by RT-PCR, respectively. A stronger correlation was observed between viral loads detected by RT3 and RT-PCR than between RT2 and RT-PCR (r = 0.95, P < 0.001, and r = 0.92, P < 0.001, respectively). The correlation between serial samples collected from 6 patients at 1, 3, 6, 12, 18, and 24 months after beginning triple combination antiretroviral therapy, using the 2 different methodologies, was also strong (r = 0.99, P < 0.001, for RT3 and RT-PCR, r = 0.98, P < 0.001, for RT2 and RT-PCR). The viral loads detected by RT activity assay were inversely correlated with CD4+ T-cell counts. Reproducibility of the RT activity assay was assessed by testing 3 samples in triplicate by 3 different operators. Viral load testing using assays that measure HIV RT activity is an affordable, feasible, simple, and reliable alternative for HIV RNA viral load determination in laboratories in China and other developing countries.

Original languageEnglish
Pages (from-to)208-213
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume68
Issue number3
DOIs
Publication statusPublished - Nov 2010
Externally publishedYes

Keywords

  • AIDS
  • HIV
  • HIV diagnosis test
  • HIV viral load
  • Reverse transcriptase activity
  • Treatment monitoring

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