HIV-1 predisposed to acquiring resistance to maraviroc (MVC) and other CCR5 antagonists in vitro has an inherent, low-level ability to utilize MVC-bound CCR5 for entry

Michael Roche; Martin Jakobsen; Anne Ellett; Hamid Salimiseyedabad; Becky Jubb; Mike Westby; Benhur Lee; Sharon Ruth Lewin; Melissa Churchill; Paul R Gorry

doi:10.1186/1742-4690-8-89

HIV-1 predisposed to acquiring resistance to maraviroc (MVC) and other CCR5 antagonists in vitro has an inherent, low-level ability to utilize MVC-bound CCR5 for entry

Michael Roche, Martin Jakobsen, Anne Ellett, Hamid Salimiseyedabad, Becky Jubb, Mike Westby, Benhur Lee, Sharon Ruth Lewin, Melissa Churchill, Paul R Gorry

Research output: Contribution to journal › Article › Research › peer-review

37 Citations (Scopus)

Abstract

Here, we show that Env derived from the parental CC1/85 strain is inherently capable of a low affinity interaction with MVC-bound CCR5. However, this phenotype was only revealed in 293-Affinofile cells and NP2-CD4/ CCR5 cells that express very high levels of CCR5, and was masked in TZM-bl, JC53 and U87-CD4/CCR5 cells as well as PBMC, which express comparatively lower levels of CCR5 and which are more commonly used to detect resistance to CCR5 antagonists.

Original language	English
Pages (from-to)	1 - 7
Number of pages	7
Journal	Retrovirology
Volume	8
Issue number	89
DOIs	https://doi.org/10.1186/1742-4690-8-89
Publication status	Published - 2011

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Roche, M., Jakobsen, M., Ellett, A., Salimiseyedabad, H., Jubb, B., Westby, M., Lee, B., Lewin, S. R., Churchill, M., & Gorry, P. R. (2011). HIV-1 predisposed to acquiring resistance to maraviroc (MVC) and other CCR5 antagonists in vitro has an inherent, low-level ability to utilize MVC-bound CCR5 for entry. Retrovirology, 8(89), 1 - 7. https://doi.org/10.1186/1742-4690-8-89

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title = "HIV-1 predisposed to acquiring resistance to maraviroc (MVC) and other CCR5 antagonists in vitro has an inherent, low-level ability to utilize MVC-bound CCR5 for entry",

abstract = "Here, we show that Env derived from the parental CC1/85 strain is inherently capable of a low affinity interaction with MVC-bound CCR5. However, this phenotype was only revealed in 293-Affinofile cells and NP2-CD4/ CCR5 cells that express very high levels of CCR5, and was masked in TZM-bl, JC53 and U87-CD4/CCR5 cells as well as PBMC, which express comparatively lower levels of CCR5 and which are more commonly used to detect resistance to CCR5 antagonists.",

author = "Michael Roche and Martin Jakobsen and Anne Ellett and Hamid Salimiseyedabad and Becky Jubb and Mike Westby and Benhur Lee and Lewin, {Sharon Ruth} and Melissa Churchill and Gorry, {Paul R}",

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doi = "10.1186/1742-4690-8-89",

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Roche, M, Jakobsen, M, Ellett, A, Salimiseyedabad, H, Jubb, B, Westby, M, Lee, B, Lewin, SR, Churchill, M & Gorry, PR 2011, 'HIV-1 predisposed to acquiring resistance to maraviroc (MVC) and other CCR5 antagonists in vitro has an inherent, low-level ability to utilize MVC-bound CCR5 for entry', Retrovirology, vol. 8, no. 89, pp. 1 - 7. https://doi.org/10.1186/1742-4690-8-89

TY - JOUR

T1 - HIV-1 predisposed to acquiring resistance to maraviroc (MVC) and other CCR5 antagonists in vitro has an inherent, low-level ability to utilize MVC-bound CCR5 for entry

AU - Roche, Michael

AU - Jakobsen, Martin

AU - Ellett, Anne

AU - Salimiseyedabad, Hamid

AU - Jubb, Becky

AU - Westby, Mike

AU - Lee, Benhur

AU - Lewin, Sharon Ruth

AU - Churchill, Melissa

AU - Gorry, Paul R

PY - 2011

Y1 - 2011

N2 - Here, we show that Env derived from the parental CC1/85 strain is inherently capable of a low affinity interaction with MVC-bound CCR5. However, this phenotype was only revealed in 293-Affinofile cells and NP2-CD4/ CCR5 cells that express very high levels of CCR5, and was masked in TZM-bl, JC53 and U87-CD4/CCR5 cells as well as PBMC, which express comparatively lower levels of CCR5 and which are more commonly used to detect resistance to CCR5 antagonists.

AB - Here, we show that Env derived from the parental CC1/85 strain is inherently capable of a low affinity interaction with MVC-bound CCR5. However, this phenotype was only revealed in 293-Affinofile cells and NP2-CD4/ CCR5 cells that express very high levels of CCR5, and was masked in TZM-bl, JC53 and U87-CD4/CCR5 cells as well as PBMC, which express comparatively lower levels of CCR5 and which are more commonly used to detect resistance to CCR5 antagonists.

UR - http://www.retrovirology.com/content/pdf/1742-4690-8-89.pdf

U2 - 10.1186/1742-4690-8-89

DO - 10.1186/1742-4690-8-89

M3 - Article

SN - 1742-4690

VL - 8

SP - 1

EP - 7

JO - Retrovirology

JF - Retrovirology

IS - 89

ER -

HIV-1 predisposed to acquiring resistance to maraviroc (MVC) and other CCR5 antagonists in vitro has an inherent, low-level ability to utilize MVC-bound CCR5 for entry

Abstract

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