TY - JOUR
T1 - HisB from Mycobacterium tuberculosis
T2 - Cloning, overexpression in Mycobacterium smegmatis, purification, crystallization and preliminary X-ray crystallographic analysis
AU - Ahangar, Mohammad Syed
AU - Khandokar, Yogesh
AU - Nasir, Nazia
AU - Vyas, Rajan
AU - Biswal, Bichitra K.
PY - 2011/11
Y1 - 2011/11
N2 - HisB, encoded by open reading frame Rv1601, possesses enzymatic activity as an imidazoleglycerol-phosphate dehydratase in the histidine-biosynthetic pathway of Mycobacterium tuberculosis. A recombinant form of HisB was crystallized in three crystal forms: crystals grown using 20% PEG 1500 as a precipitant belonged to either the cubic space group P432 or the tetragonal space group P4, while an orthorhombic crystal form belonging to space group P21212 was obtained using 15% PEG 5000 and 10 mM MnCl2 as precipitant. The structure of HisB in the orthorhombic crystal form was solved by the molecular-replacement method using the crystal structure of its Arabidopsis thaliana counterpart, which shares 47% sequence identity with Rv1601, as the search model.
AB - HisB, encoded by open reading frame Rv1601, possesses enzymatic activity as an imidazoleglycerol-phosphate dehydratase in the histidine-biosynthetic pathway of Mycobacterium tuberculosis. A recombinant form of HisB was crystallized in three crystal forms: crystals grown using 20% PEG 1500 as a precipitant belonged to either the cubic space group P432 or the tetragonal space group P4, while an orthorhombic crystal form belonging to space group P21212 was obtained using 15% PEG 5000 and 10 mM MnCl2 as precipitant. The structure of HisB in the orthorhombic crystal form was solved by the molecular-replacement method using the crystal structure of its Arabidopsis thaliana counterpart, which shares 47% sequence identity with Rv1601, as the search model.
KW - HisB
KW - Mycobacterium tuberculosis
KW - Rv1601
UR - http://www.scopus.com/inward/record.url?scp=80955155508&partnerID=8YFLogxK
U2 - 10.1107/S1744309111037201
DO - 10.1107/S1744309111037201
M3 - Article
AN - SCOPUS:80955155508
SN - 1744-3091
VL - 67
SP - 1451
EP - 1456
JO - Acta Crystallographica Section F: Structural Biology Communications
JF - Acta Crystallographica Section F: Structural Biology Communications
IS - 11
ER -