Highly coordinated proteome dynamics during reprogramming of somatic cells to pluripotency

Jenny Hansson, Mahmoud Reza Rafiee, Sonja Reiland, Jose Maria Polo, Julian Gehring, Satoshi Okawa, Wolfgang Huber, Konrad Hochedlinger, Jeroen Krijgsveld

    Research output: Contribution to journalArticleResearchpeer-review

    158 Citations (Scopus)

    Abstract

    Generation of induced pluripotent stem cells (iPSCs) is a process whose mechanistic underpinnings are only beginning to emerge. Here, we applied in-depth quantitative proteomics to monitor proteome changes during the course of reprogramming of fibroblasts to iPSCs. We uncover a two-step resetting of the proteome during the first and last 3 days of reprogramming, with multiple functionally related proteins changing in expression in a highly coordinated fashion. This comprised several biological processes, including changes in the stoichiometry of electron transport-chain complexes, repressed vesicle-mediated transport during the intermediate stage, and an EMT-like process in the late phase. In addition, we demonstrate that the nucleoporin Nup210 is essential for reprogramming by its permitting of rapid cellular proliferation and subsequent progression through MET. Along with the identification of proteins expressed in a stage-specific manner, this study provides a rich resource toward an enhanced mechanistic understanding of cellular reprogramming.
    Original languageEnglish
    Pages (from-to)1579 - 1592
    Number of pages14
    JournalCell Reports
    Volume2
    Issue number6
    DOIs
    Publication statusPublished - 2012

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