TY - JOUR
T1 - Highly coordinated proteome dynamics during reprogramming of somatic cells to pluripotency
AU - Hansson, Jenny
AU - Reza Rafiee, Mahmoud
AU - Reiland, Sonja
AU - Polo, Jose Maria
AU - Gehring, Julian
AU - Okawa, Satoshi
AU - Huber, Wolfgang
AU - Hochedlinger, Konrad
AU - Krijgsveld, Jeroen
PY - 2012
Y1 - 2012
N2 - Generation of induced pluripotent stem cells (iPSCs) is a process whose mechanistic underpinnings are only beginning to emerge. Here, we applied in-depth quantitative proteomics to monitor proteome changes during the course of reprogramming of fibroblasts to iPSCs. We uncover a two-step resetting of the proteome during the first and last 3 days of reprogramming, with multiple functionally related proteins changing in expression in a highly coordinated fashion. This comprised several biological processes, including changes in the stoichiometry of electron transport-chain complexes, repressed vesicle-mediated transport during the intermediate stage, and an EMT-like process in the late phase. In addition, we demonstrate that the nucleoporin Nup210 is essential for reprogramming by its permitting of rapid cellular proliferation and subsequent progression through MET. Along with the identification of proteins expressed in a stage-specific manner, this study provides a rich resource toward an enhanced mechanistic understanding of cellular reprogramming.
AB - Generation of induced pluripotent stem cells (iPSCs) is a process whose mechanistic underpinnings are only beginning to emerge. Here, we applied in-depth quantitative proteomics to monitor proteome changes during the course of reprogramming of fibroblasts to iPSCs. We uncover a two-step resetting of the proteome during the first and last 3 days of reprogramming, with multiple functionally related proteins changing in expression in a highly coordinated fashion. This comprised several biological processes, including changes in the stoichiometry of electron transport-chain complexes, repressed vesicle-mediated transport during the intermediate stage, and an EMT-like process in the late phase. In addition, we demonstrate that the nucleoporin Nup210 is essential for reprogramming by its permitting of rapid cellular proliferation and subsequent progression through MET. Along with the identification of proteins expressed in a stage-specific manner, this study provides a rich resource toward an enhanced mechanistic understanding of cellular reprogramming.
U2 - 10.1016/j.celrep.2012.10.014
DO - 10.1016/j.celrep.2012.10.014
M3 - Article
VL - 2
SP - 1579
EP - 1592
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 6
ER -