High-titre IgM anti-sulfatide antibodies in individuals with IgM paraproteinaemia and associated peripheral neuropathy

Steven Petratos, Victor J. Turnbull, Roula Papadopoulos, Margaret Ayers, Michael F. Gonzales

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

The common association between monoclonal gammopathy and peripheral neuropathy was studied in seven patients with demyelinating polyneuropathy and IgM paraproteinaemia. Plasma samples from these individuals were thoroughly tested for antiperipheral nerve myelin (PNM) antibodies and then screened for glycoprotein and glycolipid reactivity by western immunoblotting and thin-layer chromatography (TLC) immunostaining. Three of the seven samples showed strong IgM anti-PNM and antisulfatide (GalS) antibody reactivity. Two of these three plasma samples showed extraordinarily high antisulfatide IgM antibody titres, ranging from 1 x 104 to 1 x 106 arbitrary units/L. These same samples also showed intense myelin staining of sciatic nerve sections (paraffin and cryostat) and teased nerve fibres. No axonal immunoreactivity was observed. These results suggest that high titre IgM antisulfatide antibodies may play a pathogenetic role in the immune demyelination associated with IgM paraproteinaemia.

Original languageEnglish
Pages (from-to)124-132
Number of pages9
JournalImmunology and Cell Biology
Volume78
Issue number2
DOIs
Publication statusPublished - 4 May 2000
Externally publishedYes

Keywords

  • Antibodies
  • Demyelination
  • Immunoglobulin M paraproteinaemia
  • Peripheral neuropathy
  • Sulfatide

Cite this