High-throughput production and structural characterization of libraries of self-assembly lipidic cubic phase materials

Connie Darmanin, Charlotte E Conn, Janet Newman, Xavier Mulet, Shane A Seabrook, Yi Lynn Liang, Adrian Hawley, Nigel Kirby, Joseph N. Varghese, Calum J. Drummond

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36 Citations (Scopus)

Abstract

A protocol is presented for the high-throughput (HT) production of lyotropic liquid crystalline phases from libraries of lipids and lipid mixtures using standard liquid dispensing robotics, implementing methods that circumvent the problems traditionally associated with handling the highly viscous cubic phase. In addition, the ability to structurally characterize lipidic phases and assess functionality for membrane proteins contained within cubic phases, in a HT manner, is demonstrated. The techniques are combined and exemplified using the application of membrane protein crystallization within lipidic cubic phases.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalACS Combinatorial Science
Volume14
Issue number4
DOIs
Publication statusPublished - 9 Apr 2012

Keywords

  • bicontinuous cubic phase
  • high-throughput
  • in meso crystallization
  • lipid self-assembly
  • membrane proteins
  • monoolein

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