Projects per year
Hypothesis: Protein nanoparticles have attracted increased interest due to their broad applications ranging from drug delivery and vaccines to biocatalysts and biosensors. The morphology and the size of the nanoparticles play a crucial role in determining their suitability for different applications. Yet, effectively controlling the size of the nanoparticles is still a significant challenge in their manufacture. The hypothesis of this paper is that the assembly conditions and size of protein particles can be tuned via a mechanical route by simply modifying the mixing time and strength, while keeping the chemical parameters constant. Experimental: We use an acoustically actuated, high throughput, ultrafast, microfluidic mixer for the assembly of protein particles with tuneable sizes. The performance of the acoustic micro-mixer is characterized via Laser Doppler Vibrometry and image processing. The assembly of protein nanoparticles is monitored by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Findings: By changing actuation parameters, the turbulence and mixing in the microchannel can be precisely varied to control the initiation of protein particle assembly while the solution conditions of assembly (pH and ionic strength) are kept constant. Importantly, mixing times as low as 6 ms can be achieved for triggering protein assembly in the microfluidic channel. In comparison to the conventional batch process of assembly, the acoustic microfluidic mixer approach produces smaller particles with a more uniform size distribution, promising a new way to manufacture protein particles with controllable quality.
- Acoustic microfluidic mixer
- Protein nanoparticles
- Size distribution
Wang, H., Zhang, X., Duke, M. C., Hill, M., Leslie, G. L., Diniz da Costa, J. C., Gray, S. R., Nghiem, L., Millar, G., Kong, L., Liu, S., Shon, H. K., Le-Clech, P., Zhang, L., Hoadley, A., Smart, S., Dumee, L., Thornton, A. W., Ozcakmak, B., Bury, P., Stephen, G., Fane, A., Elimelech, M., Pan, B., Tao, S., Horton, A., Wu, X., Forbes, M., Hu, J., Song, H., Dixon, I., Holt, S., He, L., Williams, C., Hou, H., Shen, W., Wang, J., Gao, L., Davis, P., Pham, H., Muthukumarn, S., Bustamante, H. & Thang, S. H.
Barwon Region Water Corporation (trading as Barwon Water) (Victoria) , South East Water Corporation (trading as South East Water Ltd (SEWL) (Victoria), Tasmanian Water and Sewerage Corporation Pty Ltd, Advanced Fuel Innovation Pty Ltd, CSL Behring (Australia), Yale University, 2D Water Pty Ltd, Activated Water Technologies Pty Ltd, Akvotek Pty Ltd, Bioactive Materials Pty Ltd, Australian Nuclear Science and Technology Organisation (ANSTO) , Arrow Bowen Pipeline Pty Ltd (trading as Arrow Energy), Baosteel Group Corporation, Ironwood Clean Energy Technologies Pty Limited (trading as: Carbon Technologies Australia), Shenzhen Innova Nanobody Company, Shanghai Boiler Works Co Limited, Shijiazhuang Chang’an Yucai Building Materials Ltd. Company, Zeolite Australia Pty Limited, University of Technology (UTS) Sydney
15/12/17 → 15/12/23
A novel outer-selective hollow fibre nanocomposite membranes tailored for osmotic membrane bioreactor technology: alternative technology for high quality water reuse
Shon , H. & Zhang, X.
3/01/20 → 15/12/22
Nghiem, L., Zhang, X., Elimelech, M. & Leong, S.
1/07/19 → 31/12/21