High-salt diet reveals the hypertensive and renal effects of reduced nephron endowment

Leah-Anne Maree Ruta, Hayley Dickinson, Merlin C Thomas, Katherine Maude Denton, Warwick Peter Anderson, Michelle Monica Kett

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The extent a reduced nephron endowment contributes to hypertension and renal disease is confounded in models created by intrauterine insults that also demonstrate other phenotypes. Further, recent data suggests that a reduced nephron endowment provides the first hit and simply increases the susceptibility to injurious stimuli. Thus we examined nephron number, glomerular volume, conscious mean arterial pressure (MAP) and renal function in a genetic model of reduced nephron endowment before and after a high salt (5 ) diet. One yr old GDNF wildtype (WT), heterozygous mice born with 2 kidneys (HET2K) and heterozygous mice born with one kidney (HET1K) were used. Nephron number was 25 lower in HET2K and 65 lower in HET1K mice compared to WT. Glomeruli hypertrophied in both HET groups by 33 resulting in total glomerular volumes that were similar between HET2K and WT, but remained 50 lower in HET1K mice. On normal salt, 24h MAP was not different between WT, HET2K and HET1K mice (102+/-1, 103+/-1, 102+/-2mmHg). On high salt diet, MAP increased 9.1+/-1.9mmHg in HET1K (P
Original languageEnglish
Pages (from-to)F1384 - F1392
Number of pages9
JournalAmerican Journal of Physiology-Renal Physiology
Issue number6
Publication statusPublished - 2010

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