High-Resolution X-ray Phase-Contrast 3D Imaging of Breast Tissue Specimens as a Possible Adjunct to Histopathology

Patrycja Baran, Sheridan Mayo, Mikkaela McCormack, Serena Pacile, Giuliana Tromba, Christian Dullin, Fabrizio Zanconati, Fulvia Arfelli, Diego Dreossi, Jane Fox, Zdenka Prodanovic, Marian Cholewa, Harry Quiney, Matthew Dimmock, Yakov Nesterets, Darren Thompson, Patrick Brennan, Timur Gureyev

Research output: Contribution to journalArticleResearchpeer-review

Abstract

THistopathological analysis is the current gold standard in breast cancer diagnosis and management, however, as imaging technology improves, the amount of potential diagnostic information that may be demonstrable radiologically should also increase. We aimed to evaluate the potential clinical usefulness of 3D phase-contrast micro-computed tomography (micro-CT) imaging at high spatial resolutions as an adjunct to conventional histological microscopy. Ten breast tissue specimens, 2 mm in diameter, were scanned at the SYRMEP beamline of the Elettra Synchrotron using the propagation-based phase-contrast micro-tomography method. We obtained 1.2 ìm pixel size images, which were analysed and compared with corresponding histological sections examined under light microscopy. To evaluate the effect of spatial resolution on breast cancer diagnosis, scans with 4 different pixel sizes were also performed. Our comparative analysis revealed that high-resolution images can enable, at a near-histological level, detailed architectural assessment of tissue that may permit increased breast cancer diagnostic sensitivity and specificity when compared with current imaging practices. The potential clinical applications of this method are also discussed.

Original languageEnglish
Number of pages10
JournalIEEE Transactions on Medical Imaging
DOIs
Publication statusAccepted/In press - 9 Jun 2018

Keywords

  • breast cancer
  • computed tomography
  • histology
  • phase contrast
  • X-ray imaging

Cite this

Baran, Patrycja ; Mayo, Sheridan ; McCormack, Mikkaela ; Pacile, Serena ; Tromba, Giuliana ; Dullin, Christian ; Zanconati, Fabrizio ; Arfelli, Fulvia ; Dreossi, Diego ; Fox, Jane ; Prodanovic, Zdenka ; Cholewa, Marian ; Quiney, Harry ; Dimmock, Matthew ; Nesterets, Yakov ; Thompson, Darren ; Brennan, Patrick ; Gureyev, Timur. / High-Resolution X-ray Phase-Contrast 3D Imaging of Breast Tissue Specimens as a Possible Adjunct to Histopathology. In: IEEE Transactions on Medical Imaging. 2018.
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abstract = "THistopathological analysis is the current gold standard in breast cancer diagnosis and management, however, as imaging technology improves, the amount of potential diagnostic information that may be demonstrable radiologically should also increase. We aimed to evaluate the potential clinical usefulness of 3D phase-contrast micro-computed tomography (micro-CT) imaging at high spatial resolutions as an adjunct to conventional histological microscopy. Ten breast tissue specimens, 2 mm in diameter, were scanned at the SYRMEP beamline of the Elettra Synchrotron using the propagation-based phase-contrast micro-tomography method. We obtained 1.2 {\`i}m pixel size images, which were analysed and compared with corresponding histological sections examined under light microscopy. To evaluate the effect of spatial resolution on breast cancer diagnosis, scans with 4 different pixel sizes were also performed. Our comparative analysis revealed that high-resolution images can enable, at a near-histological level, detailed architectural assessment of tissue that may permit increased breast cancer diagnostic sensitivity and specificity when compared with current imaging practices. The potential clinical applications of this method are also discussed.",
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author = "Patrycja Baran and Sheridan Mayo and Mikkaela McCormack and Serena Pacile and Giuliana Tromba and Christian Dullin and Fabrizio Zanconati and Fulvia Arfelli and Diego Dreossi and Jane Fox and Zdenka Prodanovic and Marian Cholewa and Harry Quiney and Matthew Dimmock and Yakov Nesterets and Darren Thompson and Patrick Brennan and Timur Gureyev",
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Baran, P, Mayo, S, McCormack, M, Pacile, S, Tromba, G, Dullin, C, Zanconati, F, Arfelli, F, Dreossi, D, Fox, J, Prodanovic, Z, Cholewa, M, Quiney, H, Dimmock, M, Nesterets, Y, Thompson, D, Brennan, P & Gureyev, T 2018, 'High-Resolution X-ray Phase-Contrast 3D Imaging of Breast Tissue Specimens as a Possible Adjunct to Histopathology' IEEE Transactions on Medical Imaging. https://doi.org/10.1109/TMI.2018.2845905

High-Resolution X-ray Phase-Contrast 3D Imaging of Breast Tissue Specimens as a Possible Adjunct to Histopathology. / Baran, Patrycja; Mayo, Sheridan; McCormack, Mikkaela; Pacile, Serena; Tromba, Giuliana; Dullin, Christian; Zanconati, Fabrizio; Arfelli, Fulvia; Dreossi, Diego; Fox, Jane; Prodanovic, Zdenka; Cholewa, Marian; Quiney, Harry; Dimmock, Matthew; Nesterets, Yakov; Thompson, Darren; Brennan, Patrick; Gureyev, Timur.

In: IEEE Transactions on Medical Imaging, 09.06.2018.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Baran, Patrycja

AU - Mayo, Sheridan

AU - McCormack, Mikkaela

AU - Pacile, Serena

AU - Tromba, Giuliana

AU - Dullin, Christian

AU - Zanconati, Fabrizio

AU - Arfelli, Fulvia

AU - Dreossi, Diego

AU - Fox, Jane

AU - Prodanovic, Zdenka

AU - Cholewa, Marian

AU - Quiney, Harry

AU - Dimmock, Matthew

AU - Nesterets, Yakov

AU - Thompson, Darren

AU - Brennan, Patrick

AU - Gureyev, Timur

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N2 - THistopathological analysis is the current gold standard in breast cancer diagnosis and management, however, as imaging technology improves, the amount of potential diagnostic information that may be demonstrable radiologically should also increase. We aimed to evaluate the potential clinical usefulness of 3D phase-contrast micro-computed tomography (micro-CT) imaging at high spatial resolutions as an adjunct to conventional histological microscopy. Ten breast tissue specimens, 2 mm in diameter, were scanned at the SYRMEP beamline of the Elettra Synchrotron using the propagation-based phase-contrast micro-tomography method. We obtained 1.2 ìm pixel size images, which were analysed and compared with corresponding histological sections examined under light microscopy. To evaluate the effect of spatial resolution on breast cancer diagnosis, scans with 4 different pixel sizes were also performed. Our comparative analysis revealed that high-resolution images can enable, at a near-histological level, detailed architectural assessment of tissue that may permit increased breast cancer diagnostic sensitivity and specificity when compared with current imaging practices. The potential clinical applications of this method are also discussed.

AB - THistopathological analysis is the current gold standard in breast cancer diagnosis and management, however, as imaging technology improves, the amount of potential diagnostic information that may be demonstrable radiologically should also increase. We aimed to evaluate the potential clinical usefulness of 3D phase-contrast micro-computed tomography (micro-CT) imaging at high spatial resolutions as an adjunct to conventional histological microscopy. Ten breast tissue specimens, 2 mm in diameter, were scanned at the SYRMEP beamline of the Elettra Synchrotron using the propagation-based phase-contrast micro-tomography method. We obtained 1.2 ìm pixel size images, which were analysed and compared with corresponding histological sections examined under light microscopy. To evaluate the effect of spatial resolution on breast cancer diagnosis, scans with 4 different pixel sizes were also performed. Our comparative analysis revealed that high-resolution images can enable, at a near-histological level, detailed architectural assessment of tissue that may permit increased breast cancer diagnostic sensitivity and specificity when compared with current imaging practices. The potential clinical applications of this method are also discussed.

KW - breast cancer

KW - computed tomography

KW - histology

KW - phase contrast

KW - X-ray imaging

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M3 - Article

JO - IEEE Transactions on Medical Imaging

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