TY - JOUR
T1 - High-resolution light-sheet microscopy for whole-cell sub-cellular dynamics
AU - Kreplin, Laura Zoe
AU - Arumugam, Senthil
N1 - Funding Information:
S.A. thanks the National Health and Medical Research Council of Australia (APP1182212). The EMBL Australia Partnership Laboratory (EMBL Australia) is supported by the National Collaborative Research Infrastructure Strategy of the Australian Government. LZK thanks the Monash Biomedicine Discovery Institute PhD scholarship. The authors thank Harrison M York for discussions and the anonymous reviewer for critical feedback.
Funding Information:
S.A. thanks the National Health and Medical Research Council of Australia (APP1182212). The EMBL Australia Partnership Laboratory ( EMBL Australia ) is supported by the National Collaborative Research Infrastructure Strategy of the Australian Government . LZK thanks the Monash Biomedicine Discovery Institute PhD scholarship. The authors thank Harrison M York for discussions and the anonymous reviewer for critical feedback.
Publisher Copyright:
© 2023 Elsevier Ltd
PY - 2023/12
Y1 - 2023/12
N2 - Research in the areas of organelle dynamics, cytoskeletal interactions, membrane protrusions, and cell motility relies heavily on live-cell imaging. These structures continuously move about in complex patterns and imaging them live at sufficient temporal resolutions as well as for durations long enough to extract significant number of events is an absolute necessity. Capturing most of the sub-cellular dynamics in whole cell volumes was beyond reach due to the lack of balance between reduced photo-toxicity, time resolution, and the required spatial resolution in dominant imaging modalities like point scanning confocal and spinning disc confocal microscopy. In the last few years, a plethora of light-sheet geometries have emerged, pushing the limits of measurements. In this review, we will focus on a subset of light-sheet modalities that are most suited to studying live, sub-cellular dynamics in whole-cell volumes.
AB - Research in the areas of organelle dynamics, cytoskeletal interactions, membrane protrusions, and cell motility relies heavily on live-cell imaging. These structures continuously move about in complex patterns and imaging them live at sufficient temporal resolutions as well as for durations long enough to extract significant number of events is an absolute necessity. Capturing most of the sub-cellular dynamics in whole cell volumes was beyond reach due to the lack of balance between reduced photo-toxicity, time resolution, and the required spatial resolution in dominant imaging modalities like point scanning confocal and spinning disc confocal microscopy. In the last few years, a plethora of light-sheet geometries have emerged, pushing the limits of measurements. In this review, we will focus on a subset of light-sheet modalities that are most suited to studying live, sub-cellular dynamics in whole-cell volumes.
UR - http://www.scopus.com/inward/record.url?scp=85175049172&partnerID=8YFLogxK
U2 - 10.1016/j.ceb.2023.102272
DO - 10.1016/j.ceb.2023.102272
M3 - Review Article
C2 - 39491307
AN - SCOPUS:85175049172
SN - 0955-0674
VL - 85
JO - Current Opinion in Cell Biology
JF - Current Opinion in Cell Biology
M1 - 102272
ER -