High-resolution cryo-EM: the nuts and bolts

Research output: Contribution to journalReview ArticleResearchpeer-review

Abstract

Cryogenic electron microscopy (cryo-EM) and single-particle analysis now enables the determination of high-resolution structures of macromolecular assemblies that have resisted X-ray crystallography and other approaches. Successful high-resolution structure determination by cryo-EM always depends on the quality of the protein sample. While structural heterogeneity remains a key challenge for cryo-EM, it also represents a rare opportunity to study the intrinsic conformational flexibility of macromolecular assemblies. Here, we review the key technological advancements that have made this ‘resolution revolution’ possible and give a concise overview of the technical challenges that needed to be overcome to allow high-resolution structure determination.

Original languageEnglish
Number of pages6
JournalCurrent Opinion in Structural Biology
Volume46
DOIs
Publication statusPublished - 1 Oct 2017

Cite this

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title = "High-resolution cryo-EM: the nuts and bolts",
abstract = "Cryogenic electron microscopy (cryo-EM) and single-particle analysis now enables the determination of high-resolution structures of macromolecular assemblies that have resisted X-ray crystallography and other approaches. Successful high-resolution structure determination by cryo-EM always depends on the quality of the protein sample. While structural heterogeneity remains a key challenge for cryo-EM, it also represents a rare opportunity to study the intrinsic conformational flexibility of macromolecular assemblies. Here, we review the key technological advancements that have made this ‘resolution revolution’ possible and give a concise overview of the technical challenges that needed to be overcome to allow high-resolution structure determination.",
author = "Dominika Elmlund and Le, {Sarah N} and Hans Elmlund",
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doi = "10.1016/j.sbi.2017.03.003",
language = "English",
volume = "46",
journal = "Current Opinion in Structural Biology",
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High-resolution cryo-EM : the nuts and bolts. / Elmlund, Dominika; Le, Sarah N; Elmlund, Hans.

In: Current Opinion in Structural Biology, Vol. 46, 01.10.2017.

Research output: Contribution to journalReview ArticleResearchpeer-review

TY - JOUR

T1 - High-resolution cryo-EM

T2 - the nuts and bolts

AU - Elmlund, Dominika

AU - Le, Sarah N

AU - Elmlund, Hans

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Cryogenic electron microscopy (cryo-EM) and single-particle analysis now enables the determination of high-resolution structures of macromolecular assemblies that have resisted X-ray crystallography and other approaches. Successful high-resolution structure determination by cryo-EM always depends on the quality of the protein sample. While structural heterogeneity remains a key challenge for cryo-EM, it also represents a rare opportunity to study the intrinsic conformational flexibility of macromolecular assemblies. Here, we review the key technological advancements that have made this ‘resolution revolution’ possible and give a concise overview of the technical challenges that needed to be overcome to allow high-resolution structure determination.

AB - Cryogenic electron microscopy (cryo-EM) and single-particle analysis now enables the determination of high-resolution structures of macromolecular assemblies that have resisted X-ray crystallography and other approaches. Successful high-resolution structure determination by cryo-EM always depends on the quality of the protein sample. While structural heterogeneity remains a key challenge for cryo-EM, it also represents a rare opportunity to study the intrinsic conformational flexibility of macromolecular assemblies. Here, we review the key technological advancements that have made this ‘resolution revolution’ possible and give a concise overview of the technical challenges that needed to be overcome to allow high-resolution structure determination.

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U2 - 10.1016/j.sbi.2017.03.003

DO - 10.1016/j.sbi.2017.03.003

M3 - Review Article

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SN - 0959-440X

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