High ratios of C20: 4n-6/C20:5n-3 and thromboxane B2/6-keto-prostaglandin F in placenta are potential Risk contributors for neural Tube Defects: A case-Control study in Shanxi Province, China

Kelei Li, Xiaotian Zhang, Lijun Pei, Gong Chen, Jianmeng Liu, Mark L. Wahlqvist, Xiaoying Zheng, Duo Li

Research output: Contribution to journalArticleOtherpeer-review

Abstract

Background: Neural tube defects (NTDs) are severe congenital malformations. Folate supplementation can reduce the risk, but cannot prevent all NTDs, suggesting other reasons for folate-resistant NTDs. The present study assesses placental fatty acid composition, eicosanoids, and cytokines as risk factors for NTDs in a Chinese population with highly incident NTDs. Methods: Seventy-seven aborted fetuses with NTDs during the third trimester were cases and 142 healthy newborns were controls. Placental fatty acid composition, eicosanoids, and cytokines were determined by standard methods. Results: The placental C20:4n-6/C20:5n-3 and thromboxane B2 (TXB2)/6-keto-prostaglandin F1α (6-keto-PGF1α) ratios were significantly higher for cases than controls (p < 0.001 and 0.05, respectively). For the top versus the lowest tertiles of placental C20:4n-6/C20:5n-3 and TXB2/6-keto-PGF1α, odds ratios for NTD occurrence were 3.79 (95% confidence interval, 1.60-8.96) (p for trend < 0.01) and 5.52 (95% confidence interval, 2.07-14.74) (p for trend < 0.001), respectively, adjusted for fetal sex as well as maternal age, occupation, parity, smoking, passive smoking, periconceptional folate supplementation, conception season, and tea drinking. The C20:4n-6/C20:5n-3 and TXB2/6-keto-PGF1α ratios were positively correlated (r = 0.14; p < 0.05). The proportions of C18:2n-6, C18:3n-6, C20:3n-6, C18:3n-3, C20:3n-3, C20:5n-3, and C22:5n-3 were significantly lower in cases than controls, and all negatively associated with NTD occurrence (tertile-specific odds ratios); after adjustment for the potential confounders, these associations remained significant (p for trend < 0.05) except for C20:3n-3. Conclusion: High placental ratios of C20:4n-6/C20:5n-3 and TXB2/6-keto-PGF1α are risk factors for neural tube defects.

Original languageEnglish
Pages (from-to)550-563
Number of pages14
JournalBirth Defects Research
Volume109
Issue number8
DOIs
Publication statusPublished - 2017

Keywords

  • Cytokines
  • Eicosanoids
  • Fatty acids
  • Human
  • Placenta

Cite this

@article{f6472d33f70e472ba955e94bd8abe73d,
title = "High ratios of C20:: 4n-6/C20:5n-3 and thromboxane B2/6-keto-prostaglandin F1α in placenta are potential Risk contributors for neural Tube Defects: A case-Control study in Shanxi Province, China",
abstract = "Background: Neural tube defects (NTDs) are severe congenital malformations. Folate supplementation can reduce the risk, but cannot prevent all NTDs, suggesting other reasons for folate-resistant NTDs. The present study assesses placental fatty acid composition, eicosanoids, and cytokines as risk factors for NTDs in a Chinese population with highly incident NTDs. Methods: Seventy-seven aborted fetuses with NTDs during the third trimester were cases and 142 healthy newborns were controls. Placental fatty acid composition, eicosanoids, and cytokines were determined by standard methods. Results: The placental C20:4n-6/C20:5n-3 and thromboxane B2 (TXB2)/6-keto-prostaglandin F1α (6-keto-PGF1α) ratios were significantly higher for cases than controls (p < 0.001 and 0.05, respectively). For the top versus the lowest tertiles of placental C20:4n-6/C20:5n-3 and TXB2/6-keto-PGF1α, odds ratios for NTD occurrence were 3.79 (95{\%} confidence interval, 1.60-8.96) (p for trend < 0.01) and 5.52 (95{\%} confidence interval, 2.07-14.74) (p for trend < 0.001), respectively, adjusted for fetal sex as well as maternal age, occupation, parity, smoking, passive smoking, periconceptional folate supplementation, conception season, and tea drinking. The C20:4n-6/C20:5n-3 and TXB2/6-keto-PGF1α ratios were positively correlated (r = 0.14; p < 0.05). The proportions of C18:2n-6, C18:3n-6, C20:3n-6, C18:3n-3, C20:3n-3, C20:5n-3, and C22:5n-3 were significantly lower in cases than controls, and all negatively associated with NTD occurrence (tertile-specific odds ratios); after adjustment for the potential confounders, these associations remained significant (p for trend < 0.05) except for C20:3n-3. Conclusion: High placental ratios of C20:4n-6/C20:5n-3 and TXB2/6-keto-PGF1α are risk factors for neural tube defects.",
keywords = "Cytokines, Eicosanoids, Fatty acids, Human, Placenta",
author = "Kelei Li and Xiaotian Zhang and Lijun Pei and Gong Chen and Jianmeng Liu and Wahlqvist, {Mark L.} and Xiaoying Zheng and Duo Li",
year = "2017",
doi = "10.1002/bdr2.1003",
language = "English",
volume = "109",
pages = "550--563",
journal = "Birth Defects Research",
issn = "2472-1727",
publisher = "Wiley-Blackwell",
number = "8",

}

High ratios of C20: 4n-6/C20:5n-3 and thromboxane B2/6-keto-prostaglandin F in placenta are potential Risk contributors for neural Tube Defects: A case-Control study in Shanxi Province, China. / Li, Kelei; Zhang, Xiaotian; Pei, Lijun; Chen, Gong; Liu, Jianmeng; Wahlqvist, Mark L.; Zheng, Xiaoying; Li, Duo.

In: Birth Defects Research, Vol. 109, No. 8, 2017, p. 550-563.

Research output: Contribution to journalArticleOtherpeer-review

TY - JOUR

T1 - High ratios of C20:

T2 - 4n-6/C20:5n-3 and thromboxane B2/6-keto-prostaglandin F1α in placenta are potential Risk contributors for neural Tube Defects: A case-Control study in Shanxi Province, China

AU - Li, Kelei

AU - Zhang, Xiaotian

AU - Pei, Lijun

AU - Chen, Gong

AU - Liu, Jianmeng

AU - Wahlqvist, Mark L.

AU - Zheng, Xiaoying

AU - Li, Duo

PY - 2017

Y1 - 2017

N2 - Background: Neural tube defects (NTDs) are severe congenital malformations. Folate supplementation can reduce the risk, but cannot prevent all NTDs, suggesting other reasons for folate-resistant NTDs. The present study assesses placental fatty acid composition, eicosanoids, and cytokines as risk factors for NTDs in a Chinese population with highly incident NTDs. Methods: Seventy-seven aborted fetuses with NTDs during the third trimester were cases and 142 healthy newborns were controls. Placental fatty acid composition, eicosanoids, and cytokines were determined by standard methods. Results: The placental C20:4n-6/C20:5n-3 and thromboxane B2 (TXB2)/6-keto-prostaglandin F1α (6-keto-PGF1α) ratios were significantly higher for cases than controls (p < 0.001 and 0.05, respectively). For the top versus the lowest tertiles of placental C20:4n-6/C20:5n-3 and TXB2/6-keto-PGF1α, odds ratios for NTD occurrence were 3.79 (95% confidence interval, 1.60-8.96) (p for trend < 0.01) and 5.52 (95% confidence interval, 2.07-14.74) (p for trend < 0.001), respectively, adjusted for fetal sex as well as maternal age, occupation, parity, smoking, passive smoking, periconceptional folate supplementation, conception season, and tea drinking. The C20:4n-6/C20:5n-3 and TXB2/6-keto-PGF1α ratios were positively correlated (r = 0.14; p < 0.05). The proportions of C18:2n-6, C18:3n-6, C20:3n-6, C18:3n-3, C20:3n-3, C20:5n-3, and C22:5n-3 were significantly lower in cases than controls, and all negatively associated with NTD occurrence (tertile-specific odds ratios); after adjustment for the potential confounders, these associations remained significant (p for trend < 0.05) except for C20:3n-3. Conclusion: High placental ratios of C20:4n-6/C20:5n-3 and TXB2/6-keto-PGF1α are risk factors for neural tube defects.

AB - Background: Neural tube defects (NTDs) are severe congenital malformations. Folate supplementation can reduce the risk, but cannot prevent all NTDs, suggesting other reasons for folate-resistant NTDs. The present study assesses placental fatty acid composition, eicosanoids, and cytokines as risk factors for NTDs in a Chinese population with highly incident NTDs. Methods: Seventy-seven aborted fetuses with NTDs during the third trimester were cases and 142 healthy newborns were controls. Placental fatty acid composition, eicosanoids, and cytokines were determined by standard methods. Results: The placental C20:4n-6/C20:5n-3 and thromboxane B2 (TXB2)/6-keto-prostaglandin F1α (6-keto-PGF1α) ratios were significantly higher for cases than controls (p < 0.001 and 0.05, respectively). For the top versus the lowest tertiles of placental C20:4n-6/C20:5n-3 and TXB2/6-keto-PGF1α, odds ratios for NTD occurrence were 3.79 (95% confidence interval, 1.60-8.96) (p for trend < 0.01) and 5.52 (95% confidence interval, 2.07-14.74) (p for trend < 0.001), respectively, adjusted for fetal sex as well as maternal age, occupation, parity, smoking, passive smoking, periconceptional folate supplementation, conception season, and tea drinking. The C20:4n-6/C20:5n-3 and TXB2/6-keto-PGF1α ratios were positively correlated (r = 0.14; p < 0.05). The proportions of C18:2n-6, C18:3n-6, C20:3n-6, C18:3n-3, C20:3n-3, C20:5n-3, and C22:5n-3 were significantly lower in cases than controls, and all negatively associated with NTD occurrence (tertile-specific odds ratios); after adjustment for the potential confounders, these associations remained significant (p for trend < 0.05) except for C20:3n-3. Conclusion: High placental ratios of C20:4n-6/C20:5n-3 and TXB2/6-keto-PGF1α are risk factors for neural tube defects.

KW - Cytokines

KW - Eicosanoids

KW - Fatty acids

KW - Human

KW - Placenta

UR - http://www.scopus.com/inward/record.url?scp=85024486144&partnerID=8YFLogxK

U2 - 10.1002/bdr2.1003

DO - 10.1002/bdr2.1003

M3 - Article

VL - 109

SP - 550

EP - 563

JO - Birth Defects Research

JF - Birth Defects Research

SN - 2472-1727

IS - 8

ER -