High rates of survival and fertilization of mouse and hamster oocytes after vitrification in dimethylsulphoxide

M. J. Wood, C. Barros, C. J. Candy, J. Carroll, J. Melendez, D. G. Whittingham

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Abstract

A high proportion (> 70%) of mouse and hamster oocytes exposed for 3-5 min to 1.5 M dimethylsulfoxide (DMSO) and washed briefly in 3.9 M DMSO before vitrification in 6.0 M DMSO appeared morphologically normal on recovery. Significantly fewer (< 46%) mouse oocytes appeared normal when the time of exposure to 1.5 M DMSO was reduced to 1 min or less. The rate of fertilization in vitro of vitrified oocytes was reduced compared to the rate for untreated controls (mouse: 79% vs. 94%; hamster: 73% vs. 87%). After removal of the zona pellucida, fertilization was similar in vitrified and control hamster oocytes inseminated with hamster (> 90%) or human (21% vs. 23%) sperm. Sperm nuclear decondensation and pronuclear formation appeared to be delayed in the cytoplasm of vitrified hamster oocytes. Seventy-nine percent of 2-cell-stage mouse embryos derived from vitrified oocytes implanted after transfer to pseudopregnant recipients, but only 40% developed to normal fetuses compared to 61% of controls. The reason for this high rate of postimplantation loss is unknown.

Original languageEnglish
Pages (from-to)489-495
Number of pages7
JournalBiology of Reproduction
Volume49
Issue number3
DOIs
Publication statusPublished - 1 Jan 1993
Externally publishedYes

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