TY - JOUR
T1 - High rates of CTX-M group-1 extended-spectrum β-lactamases producing Escherichia coli from pets and their owners in Faisalabad, Pakistan
AU - Abbas, Ghazanfar
AU - Khan, Iahtasham
AU - Mohsin, Mashkoor
AU - Sajjad-Ur-Rahman, null
AU - Younas, Tayyaba
AU - Ali, Shahzad
N1 - Funding Information:
We thank all pet owners and veterinary professionals involved in this study for their cooperation and help in sampling. This work was supported by the Federal German Foreign Office (grant reference number AA-OR 12–370.43), BIOS FLI PAK, and the Higher Education Commission, Pakistan (NRPU Grant number 4681).
Publisher Copyright:
© 2019 Abbas et al.
PY - 2019
Y1 - 2019
N2 - Purpose: Pet animals have been considered a potential carrier of clinically important multidrug-resistant Escherichia coli. However, little is known about the role of pets as reservoirs of extended-spectrum β-lactamase (ESBL) producing E. coli in Pakistan. This study was designed to determine the prevalence and genetic relatedness of ESBL-producing multidrug-resistant E. coli in pets, their owners, and veterinary professionals. Methods: A total of 105 fecal samples were collected from dogs, cats, their owners, and veterinary professionals from veterinary clinics. Isolates of ESBL-producing E. coli were subjected to antimicrobial susceptibility testing. The presence of bla CTX-M genes and CTX-M groups I and II in multidrug-resistant E. coli was detected using PCR. Clonal diversity was checked using BOX-PCR. Results: Of the 105 fecal samples screened, 73 (69.5%) were found to contain ESBL-producing E. coli. The percentage of ESBL-producing E. coli isolates in dogs and dog owners was found to be 81.8% (18/22) and 59% (13/22), respectively. In cats, this percentage was 73.9% (17/23) and in cat owners, 56.5% (13/23). Furthermore, 80% (12/15) of E. coli isolates in veterinary professionals were ESBL producers. Of these 73 ESBL-producing E. coli isolates, 23 isolates exhibited a multidrug-resistant phenotype. The most prevalent multidrug-resistant pattern (17.4%) identified was resistant to ampicillin, cefotaxime, ciprofloxacin, and nitrofurantoin. In the multidrug-resistant E. coli, bla CTX-M was identified as the most common ESBL-producing genotype (19/23), with bla CTX-M-1 dominating in all 19 isolates. Furthermore, BOX-PCR analysis exhibited genetically diverse clonal groups among isolates of the CTX-M-1 group. Conclusion: Our results provide important baseline information on the potential burden of multidrug-resistant E. coli among companion animals in Pakistan. Further studies are needed to understand the drivers of antimicrobial resistance at human–animal–environmental intersections.
AB - Purpose: Pet animals have been considered a potential carrier of clinically important multidrug-resistant Escherichia coli. However, little is known about the role of pets as reservoirs of extended-spectrum β-lactamase (ESBL) producing E. coli in Pakistan. This study was designed to determine the prevalence and genetic relatedness of ESBL-producing multidrug-resistant E. coli in pets, their owners, and veterinary professionals. Methods: A total of 105 fecal samples were collected from dogs, cats, their owners, and veterinary professionals from veterinary clinics. Isolates of ESBL-producing E. coli were subjected to antimicrobial susceptibility testing. The presence of bla CTX-M genes and CTX-M groups I and II in multidrug-resistant E. coli was detected using PCR. Clonal diversity was checked using BOX-PCR. Results: Of the 105 fecal samples screened, 73 (69.5%) were found to contain ESBL-producing E. coli. The percentage of ESBL-producing E. coli isolates in dogs and dog owners was found to be 81.8% (18/22) and 59% (13/22), respectively. In cats, this percentage was 73.9% (17/23) and in cat owners, 56.5% (13/23). Furthermore, 80% (12/15) of E. coli isolates in veterinary professionals were ESBL producers. Of these 73 ESBL-producing E. coli isolates, 23 isolates exhibited a multidrug-resistant phenotype. The most prevalent multidrug-resistant pattern (17.4%) identified was resistant to ampicillin, cefotaxime, ciprofloxacin, and nitrofurantoin. In the multidrug-resistant E. coli, bla CTX-M was identified as the most common ESBL-producing genotype (19/23), with bla CTX-M-1 dominating in all 19 isolates. Furthermore, BOX-PCR analysis exhibited genetically diverse clonal groups among isolates of the CTX-M-1 group. Conclusion: Our results provide important baseline information on the potential burden of multidrug-resistant E. coli among companion animals in Pakistan. Further studies are needed to understand the drivers of antimicrobial resistance at human–animal–environmental intersections.
KW - Antimicrobial resistance
KW - ESBL-producing E. coli
KW - Multidrug resistance
KW - One-health
KW - Pets
KW - Zoonosis
UR - http://www.scopus.com/inward/record.url?scp=85062965045&partnerID=8YFLogxK
U2 - 10.2147/IDR.S189884
DO - 10.2147/IDR.S189884
M3 - Article
AN - SCOPUS:85062965045
SN - 1178-6973
VL - 12
SP - 571
EP - 578
JO - Infection and Drug Resistance
JF - Infection and Drug Resistance
ER -
