Abstract
A simple and rapid high-pressure liquid chromatographic procedure is reported for the simultaneous quantitative determination of propranolol and 4-hydroxypropranolol in plasma. Following an extraction the samples are chromatographed on a reversed-phase column and the components in the column effluent are detected by fluorescence monitoring. Using 1-ml plasma samples propranolol and 4-hydroxypropranolo concentrations at least as low as 1 ng/ml and 5 ng/ml, respectively, can be quantitated. The reproducibility of the method is satisfactory and no interference from endogenous plasma components or other drugs has been observed. A single plasma sample can be analyzed in approximately 20 min.
| Original language | English |
|---|---|
| Pages (from-to) | 429-436 |
| Number of pages | 8 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 145 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1 May 1978 |
Cite this
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High-pressure liquid chromatographic method for the simultaneous quantitative analysis of propranolol and 4-hydroxypropranolol in plasma. / Nation, Roger L.; Peng, Geoffrey W.; Chiou, Win L.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 145, No. 3, 01.05.1978, p. 429-436.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - High-pressure liquid chromatographic method for the simultaneous quantitative analysis of propranolol and 4-hydroxypropranolol in plasma
AU - Nation, Roger L.
AU - Peng, Geoffrey W.
AU - Chiou, Win L.
PY - 1978/5/1
Y1 - 1978/5/1
N2 - A simple and rapid high-pressure liquid chromatographic procedure is reported for the simultaneous quantitative determination of propranolol and 4-hydroxypropranolol in plasma. Following an extraction the samples are chromatographed on a reversed-phase column and the components in the column effluent are detected by fluorescence monitoring. Using 1-ml plasma samples propranolol and 4-hydroxypropranolo concentrations at least as low as 1 ng/ml and 5 ng/ml, respectively, can be quantitated. The reproducibility of the method is satisfactory and no interference from endogenous plasma components or other drugs has been observed. A single plasma sample can be analyzed in approximately 20 min.
AB - A simple and rapid high-pressure liquid chromatographic procedure is reported for the simultaneous quantitative determination of propranolol and 4-hydroxypropranolol in plasma. Following an extraction the samples are chromatographed on a reversed-phase column and the components in the column effluent are detected by fluorescence monitoring. Using 1-ml plasma samples propranolol and 4-hydroxypropranolo concentrations at least as low as 1 ng/ml and 5 ng/ml, respectively, can be quantitated. The reproducibility of the method is satisfactory and no interference from endogenous plasma components or other drugs has been observed. A single plasma sample can be analyzed in approximately 20 min.
UR - http://www.scopus.com/inward/record.url?scp=0018147020&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(00)81372-9
DO - 10.1016/S0378-4347(00)81372-9
M3 - Article
VL - 145
SP - 429
EP - 436
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 1387-2273
IS - 3
ER -