High-performance liquid chromatography of amino acids, peptides and proteins. LXXXV. Separation of isoforms of the glycoprotein hormones from human pituitary extracts

Robyn C. Johnston, Peter G. Stanton, David M. Robertson, Milton T.W. Hearn

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10 Citations (Scopus)


Procedures for the high-resolution separation of microheterogeneous forms of the glycoprotein hormones, lutropin, follitropin and thyrotropin, present in human pituitary extracts are described. The sequential use of preparative isoelectric focusing and ion-exchange high-performance liquid chromatography (HPLC) allows the further discrimination of the isoforms of these glycoprotein hormones, previously considered homogeneous on the basis of their electrophoretic mobility. The ability of ion-exchange HPLC stationary phases to probe for slight differences in charge asymmetry expressed on the surface of these proteins raises the possibility of their rapid purification via the tandem on-line use of the same ion-exchange support with several different elution systems. Retention maps, illustrating the basis of this approach, are described.

Original languageEnglish
Pages (from-to)389-398
Number of pages10
JournalJournal of Chromatography A
Issue numberC
Publication statusPublished - 26 Jun 1987

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