High-performance liquid chromatography of amino acids, peptides and proteins LXVIII1 1 Part LXVII see ref. 14.. Evaluation of retention and bandwidth relationships of peptides related to luteinising hormone-releasing hormone and growth hormone-releasing factor, separated by gradient elution reversed-phase high-performance liquid chromatography

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Abstract

The gradient-elution behaviour of six peptides related to luteinising hormone-releasing hormone and three peptides related to growth hormone-releasing factor has been investigated using a 30-nm pore diameter n-butylsilica stationary phase and water-acetonitrile mobile phases containing 0.1 % trifluoroacetic acid. The effect of gradient time and flow-rate on the relative retentions and bandwidths of these peptides have been assessed using quantitative expressions, derived from linear solvent strength theory and general plate height theory. Whilst the retention data for these hormonal peptides appear to be adequately described by gradient elution theory for the range of operational conditions used in this study, the experimental band broadening data are not in agreement in all cases with bandwidths predicted on the basis of plate theory derived for low-molecular-weight organic molecules. Possible causes of the "non-ideal" peak shapes observed for these peptides are discussed.

Original languageEnglish
Pages (from-to)31-54
Number of pages24
JournalJournal of Chromatography A
Volume359
Issue numberC
DOIs
Publication statusPublished - 1986

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