TY - JOUR
T1 - High-performance liquid chromatography of amino acids, peptides and proteins. LVII. Analysis of radioiodinated thyrotropin polypeptides by reversed-phase high-performance liquid chromatography
AU - Stanton, Peter G.
AU - Grego, Boris
AU - Hearn, Milton T W
PY - 1984/7/27
Y1 - 1984/7/27
N2 - The chromatographic behaviour of bovine and human thyrotropin, radiolabelled stoichiometrically with lactoperoxidase, on octadecylsilicas and other reversed-phase n-alkylsilicas has been investigated. As part of this investigation the effects of a variety of elution systems on resolution and recovery have been examined. Analysis of the tryptic peptides of radioiodinated bovine thyrotropin (bTSH) preparations by reversed-phase high-performance liquid chromatographic mapping methods resulted in the separation of more than fourteen major radioactive peptide components. The data indicate that bTSH is radioiodinated with unequal incorporation into both the α- and the β-subunits. Further, assessment of the level of microheterogeneity of radiolabelled bTSH preparations can be achieved with these reversed-phase techniques.
AB - The chromatographic behaviour of bovine and human thyrotropin, radiolabelled stoichiometrically with lactoperoxidase, on octadecylsilicas and other reversed-phase n-alkylsilicas has been investigated. As part of this investigation the effects of a variety of elution systems on resolution and recovery have been examined. Analysis of the tryptic peptides of radioiodinated bovine thyrotropin (bTSH) preparations by reversed-phase high-performance liquid chromatographic mapping methods resulted in the separation of more than fourteen major radioactive peptide components. The data indicate that bTSH is radioiodinated with unequal incorporation into both the α- and the β-subunits. Further, assessment of the level of microheterogeneity of radiolabelled bTSH preparations can be achieved with these reversed-phase techniques.
UR - http://www.scopus.com/inward/record.url?scp=0021284406&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(01)96412-X
DO - 10.1016/S0021-9673(01)96412-X
M3 - Article
C2 - 6480741
AN - SCOPUS:0021284406
VL - 296
SP - 189
EP - 197
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - C
ER -