High-performance liquid chromatography of amino acids, peptides and proteins. CXI. Retention behaviour of proteins with macroporous tentacle-type anion exchangers

M. T.W. Hearn, A. N. Hodder, F. W. Fang, M. I. Aguilar

Research output: Contribution to journalArticleResearchpeer-review

20 Citations (Scopus)


The chromatographic behaviour of various proteins with Fractogel-TMAE and LiChrospher-TMAE tentacle-type strong anion-exchange sorbents has been investigated. In particular, the log k versus log 1/c (where k is the median capacity factor and c is the corresponding salt concentration) dependencies for two salt systems (NaCl and KBr) have been documented. The Fractogel-TMAE sorbent exhibited retention characteristics in terms of relative protein charge dependencies (slope Zc) and affinity values (log Kc, which is log k at c = 10-6 M, where c is the concentration of the displacer ion) which were similar to the more conventional anion-exchange sorbents such as the Mono-Q sorbent. However, the Zc and log Kc values obtained with the LiChrospher-TMAE sorbent were significantly decreased for most proteins. Furthermore, proteins such as carbonic anhydrase and myoglobin were not retained at pH values up to 4 and 2 units above their pI values, respectively. These results illustrate the different adsorptive properties of the tentacle-type sorbents compared to other monolayer or polymer layer ion exchangers in terms of accessibility of the charged ligand and the underlying retention mechanism.

Original languageEnglish
Pages (from-to)117-126
Number of pages10
JournalJournal of Chromatography A
Issue numberC
Publication statusPublished - 12 Jul 1991

Cite this