High molecular weight DNA enrichment with peptide nucleic acid probes

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Abstract

Here, we describe how peptide nucleic acid (PNA) probes can be used to enrich genomic DNA fractions to facilitate downstream analysis, such as the haplotype phasing of the isolated genomic pieces. This method enriches for polymorphic regions of fragmented chromosomes by physically separating the desired sequence and flanking regions. The PNA probes used for enrichment are novel synthetic nucleic acids with highly specific targeting and hybridization properties. Using a enrichment technique, we capture high molecular weight genomic DNA using nothing more than a simple modification to standard genomic DNA extraction from blood.

Original languageEnglish
Title of host publicationHaplotyping
Subtitle of host publicationMethods and Protocols
EditorsIrene Tiemann-Boege, Andrea Betancourt
Place of PublicationNew York NY USA
PublisherHumana Press
Chapter4
Pages73-85
Number of pages13
Volume1551
ISBN (Electronic)9781493967506
ISBN (Print)9781493967483
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1551
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Haplotype enrichment
  • High molecular weight DNA
  • Human leukocyte antigen system alleles
  • Major histocompatibility complex
  • Peptide nucleic acid probes

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